Promiscuous targeting of Bacillus subtilis cell division protein DivIVA to division sites in Escherichia coli and fission yeast

David H. Edwards, Helena B. Thomaides, Jeffery Errington

    Research output: Contribution to journalArticlepeer-review

    97 Citations (Scopus)

    Abstract

    The Bacillus subtilis divIVA gene encodes a coiled-coil protein that shows weak similarity to eukaryotic tropomyosins. The protein is targeted to the sites of cell division and mature cell poles where, in B.subtilis, it controls the site specificity of cell division. Although clear homologues of DivIVA are present only in Gram-positive bacteria, and its role in division site selection is not conserved in the Gram-negative bacterium, Escherichia coli, a DivIVA-green fluorescent protein (GFP) fusion was targeted accurately to division sites and retained at the cell pole in this organism. Remarkably, the same fusion protein was also targeted to nascent division sites and growth zones in the fission yeast Schizosaccharomyces pombe, mimicking the localization of the endogenous tropomyosin-like cell division protein Cdc8p, and F-actin. The results show that a targeting signal for division sites is conserved across the eukaryote-prokaryote divide.
    Original languageEnglish
    Pages (from-to)2719-2727
    Number of pages9
    JournalEMBO Journal
    Volume19
    Issue number11
    DOIs
    Publication statusPublished - 2000

    Keywords

    • Bacillus subtilis
    • DivIVA protein
    • Escherichia coli
    • Fission yeast
    • Tropomyosin

    Fingerprint

    Dive into the research topics of 'Promiscuous targeting of Bacillus subtilis cell division protein DivIVA to division sites in Escherichia coli and fission yeast'. Together they form a unique fingerprint.

    Cite this