Proper autophagy is indispensable for angiogenesis during chick embryo development

Wen Hui Lu, Yu Xun Shi, Zheng Lai Ma, Guang Wang, Langxia Liu, Manli Chuai, Xiaoyu Song, Andrea Münsterberg, Liu Cao, Xuesong Yang (Lead / Corresponding author)

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

People have known that autophagy plays a very important role in many physiological and pathological events. But the role of autophagy on embryonic angiogenesis still remains obscure. In this study, we demonstrated that Atg7, Atg8 and Beclin1 were expressed in the plexus vessels of angiogenesis at chick yolk sac membrane and chorioallantoic membrane. Interfering in autophagy with autophagy inducer or inhibitor could restrict the angiogenesis in vivo, which might be driven by the disorder of angiogenesis-related gene expressions, and also lead to embryonic hemorrhage, which was due to imperfection cell junctions in endothelial cells including abnormal expressions of tight junction, adheren junction and desmosome genes. Using HUVECs, we revealed that cell viability and migration ability changed with the alteration of cell autophagy exposed to RAPA or 3-MA. Interestingly, tube formation assay showed that HUVECs ability of tube formation altered with the change of Atg5, Atg7 and Atg8 manipulated by the transfection of their corresponding siRNA or plasmids. Moreover, the lost cell polarity labeled by F-actin and the absenced β-catenin in RAPA-treated and 3-MA-treated cell membrane implied intracellular cytoskeleton alteration was induced by the activation and depression of autophagy. Taken together, our current experimental data reveal that autophagy is really involved in regulating angiogenesis during embryo development.

Original languageEnglish
Pages (from-to)1742-1754
Number of pages13
JournalCell Cycle
Volume15
Issue number13
Early online date10 May 2016
DOIs
Publication statusPublished - 2 Jul 2016

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Keywords

  • angiogenesis
  • Atg
  • autophagy
  • chorioallantoic membrane
  • yolk sac membrane

Cite this

Lu, W. H., Shi, Y. X., Ma, Z. L., Wang, G., Liu, L., Chuai, M., Song, X., Münsterberg, A., Cao, L., & Yang, X. (2016). Proper autophagy is indispensable for angiogenesis during chick embryo development. Cell Cycle, 15(13), 1742-1754. https://doi.org/10.1080/15384101.2016.1184803