Protease-catalyzed peptide synthesis on solid support

Rein V. Ulijn, Beatriz Baragaña, Peter J. Halling, Sabine L. Flitsch

Research output: Contribution to journalArticlepeer-review

91 Citations (Scopus)

Abstract

The direct enzymatic synthesis of peptides from amino acids is widely used as a useful alternative to chemical synthesis. However, good yields of such enzyme-catalyzed reactions require altered reaction conditions to overcome the bias for hydrolysis in aqueous medium. We argue that the synthesis/hydrolysis equilibrium can be shifted toward synthesis in aqueous medium by immobilizing the amine on solid support. In this report, we show the first examples of solid-phase peptide synthesis catalyzed by a protease in bulk aqueous buffer.

Original languageEnglish
Pages (from-to)10988-10989
Number of pages2
JournalJournal of the American Chemical Society
Volume124
Issue number37
DOIs
Publication statusPublished - 23 Aug 2002

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