Protease-catalyzed peptide synthesis on solid support

TY - JOUR

T1 - Protease-catalyzed peptide synthesis on solid support

AU - Ulijn, Rein V.

AU - Baragaña, Beatriz

AU - Halling, Peter J.

AU - Flitsch, Sabine L.

N1 - Copyright: Copyright 2011 Elsevier B.V., All rights reserved.

PY - 2002/8/23

Y1 - 2002/8/23

N2 - The direct enzymatic synthesis of peptides from amino acids is widely used as a useful alternative to chemical synthesis. However, good yields of such enzyme-catalyzed reactions require altered reaction conditions to overcome the bias for hydrolysis in aqueous medium. We argue that the synthesis/hydrolysis equilibrium can be shifted toward synthesis in aqueous medium by immobilizing the amine on solid support. In this report, we show the first examples of solid-phase peptide synthesis catalyzed by a protease in bulk aqueous buffer.

AB - The direct enzymatic synthesis of peptides from amino acids is widely used as a useful alternative to chemical synthesis. However, good yields of such enzyme-catalyzed reactions require altered reaction conditions to overcome the bias for hydrolysis in aqueous medium. We argue that the synthesis/hydrolysis equilibrium can be shifted toward synthesis in aqueous medium by immobilizing the amine on solid support. In this report, we show the first examples of solid-phase peptide synthesis catalyzed by a protease in bulk aqueous buffer.

UR - https://www.scopus.com/pages/publications/0037130703

U2 - 10.1021/ja026912d

DO - 10.1021/ja026912d

M3 - Article

C2 - 12224940

AN - SCOPUS:0037130703

SN - 0002-7863

VL - 124

SP - 10988

EP - 10989

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 37

ER -