Protein kinase C is not a downstream effector of p21ras in activated T cells

David H. Williams (Lead / Corresponding author), Melissa Woodrow, Doreen A. Cantrell, Edward J. Murray

    Research output: Contribution to journalArticle

    25 Citations (Scopus)

    Abstract

    The aim of this present study was to investigate the role of protein kinase C (PKC), downstream of p21ras, in activating interleukin‐2 (IL‐2) gene expression. It has been reported that PKC is an effector of p21ras in T cells. Data is presented, using the potent and selective PKC inhibitor Ro 31‐8425 and transient expression of a constitutively active ras mutant, which clearly shows that PKC is not downstream of p21ras in the induction of NF‐AT and AP‐1 transcriptional activity and in the expression of IL‐2 in human Jurkat T cells. Reporter gene experiments demonstrated that NF‐χB transcriptional activity is not affected by expression of activated p21ras. The signaling pathways involving PKC activation, calcium mobilization and ras activation combine to provide the necessary components for production of IL‐2 during T cell activation.

    Original languageEnglish
    Pages (from-to)42-47
    Number of pages6
    JournalEuropean Journal of Immunology
    Volume25
    Issue number1
    DOIs
    Publication statusPublished - Jan 1995

    Keywords

    • Interleukin‐2
    • p21
    • Protein kinase C
    • T cell
    • Transcription factor

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