Protein kinase C mediates platelet secretion and thrombus formation through protein kinase D2

Olga Konopatskaya, Sharon A. Matthews, Matthew T. Harper, Karen Gilio, Judith M. E. M. Cosemans, Christopher M. Williams, Maria N. Navarro, Deborah A. Carter, Johan W. M. Heemskerk, Michael Leitges, Doreen Cantrell, Alastair W. Poole

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    32 Citations (Scopus)

    Abstract

    Platelets are highly specialized blood cells critically involved in hemostasis and thrombosis. Members of the protein kinase C (PKC) family have established roles in regulating platelet function and thrombosis, but the molecular mechanisms are not clearly understood. In particular, the conventional PKC isoform, PKC alpha, is a major regulator of platelet granule secretion, but the molecular pathway from PKC alpha to secretion is not defined. Protein kinase D (PKD) is a family of 3 kinases activated by PKC, which may represent a step in the PKC signaling pathway to secretion. In the present study, we show that PKD2 is the sole PKD member regulated downstream of PKC in platelets, and that the conventional, but not novel, PKC isoforms provide the upstream signal. Platelets from a gene knock-in mouse in which 2 key phosphorylation sites in PKD2 have been mutated (Ser707Ala/Ser711Ala) show a significant reduction in agonist-induced dense granule secretion, but not in alpha-granule secretion. This deficiency in dense granule release was responsible for a reduced platelet aggregation and a marked reduction in thrombus formation. Our results show that in the molecular pathway to secretion, PKD2 is a key component of the PKC-mediated pathway to platelet activation and thrombus formation through its selective regulation of dense granule secretion. (Blood. 2011; 118(2): 416-424)

    Original languageEnglish
    Pages (from-to)416-424
    Number of pages9
    JournalBlood
    Volume118
    Issue number2
    Early online date28 Apr 2011
    DOIs
    Publication statusPublished - 14 Jul 2011

    Keywords

    • Endothelial growth factor
    • Signal transduction pathway
    • Smooth muscle cells
    • Angiotensin-II
    • Activation
    • PKD
    • Collagen
    • Alpha
    • Phosphorylation
    • Proliferation

    Cite this

    Konopatskaya, O., Matthews, S. A., Harper, M. T., Gilio, K., Cosemans, J. M. E. M., Williams, C. M., Navarro, M. N., Carter, D. A., Heemskerk, J. W. M., Leitges, M., Cantrell, D., & Poole, A. W. (2011). Protein kinase C mediates platelet secretion and thrombus formation through protein kinase D2. Blood, 118(2), 416-424. https://doi.org/10.1182/blood-2010-10-312199