Protein kinase D enzymes are dispensable for proliferation, survival and antigen receptor-regulated NFκB activity in vertebrate B-cells

Ping Liu, Andrew M. Scharenberg, Doreen A. Cantrell, Sharon A. Matthews, Richard Marais (Editor)

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    Abstract

    To investigate the importance of protein kinase D (PKD) enzymes we generated a PKD-null DT40 B-lymphocyte cell line. Previously we have shown that PKDs have an essential role in regulating class II histone deacetylases in DT40 B-cells [Matthews, S.A., Liu, P., Spitaler, M., Olson, E.N., McKinsey, T.A., Cantrell, D.A. and Scharenberg, A.M. (2006) Essential role for protein kinase D family kinases in the regulation of class II histone deacetylases in B lymphocytes. Mol. Cell Biol. 26, 1569-1577]. We now show that PKDs are also required to regulate HSP27 phosphorylation in DT40 B-cells. However, in contrast to previous observations in other cell types, PKD enzymes do not regulate basic cellular processes such as proliferation or survival responses, nor NFkappaB transcriptional activity downstream of the B cell antigen receptor. Thus, PKDs have a selective role in DT40 B-cell biology.
    Original languageEnglish
    Pages (from-to)1377-1382
    Number of pages6
    JournalFEBS Letters
    Volume581
    Issue number7
    DOIs
    Publication statusPublished - 2007

    Keywords

    • Protein kinase D
    • Proliferation
    • Survival
    • NF-kappa B
    • HSP27

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