Abstract
To investigate the importance of protein kinase D (PKD) enzymes we generated a PKD-null DT40 B-lymphocyte cell line. Previously we have shown that PKDs have an essential role in regulating class II histone deacetylases in DT40 B-cells [Matthews, S.A., Liu, P., Spitaler, M., Olson, E.N., McKinsey, T.A., Cantrell, D.A. and Scharenberg, A.M. (2006) Essential role for protein kinase D family kinases in the regulation of class II histone deacetylases in B lymphocytes. Mol. Cell Biol. 26, 1569-1577]. We now show that PKDs are also required to regulate HSP27 phosphorylation in DT40 B-cells. However, in contrast to previous observations in other cell types, PKD enzymes do not regulate basic cellular processes such as proliferation or survival responses, nor NFkappaB transcriptional activity downstream of the B cell antigen receptor. Thus, PKDs have a selective role in DT40 B-cell biology.
Original language | English |
---|---|
Pages (from-to) | 1377-1382 |
Number of pages | 6 |
Journal | FEBS Letters |
Volume | 581 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2007 |
Keywords
- Protein kinase D
- Proliferation
- Survival
- NF-kappa B
- HSP27
Fingerprint
Dive into the research topics of 'Protein kinase D enzymes are dispensable for proliferation, survival and antigen receptor-regulated NFκB activity in vertebrate B-cells'. Together they form a unique fingerprint.Prizes
-
Royal Society Dorothy Hodgkin Research Fellowship (2003-2007)
Matthews, S. (Recipient), 2003
Prize: Fellowship awarded competitively