TY - JOUR
T1 - Protein Phosphatase 2A Is the Major Enzyme in Brain that Dephosphorylates τ Protein Phosphorylated by Proline‐Directed Protein Kinases or Cyclic AMP‐Dependent Protein Kinase
AU - Goedert, M.
AU - Jakes, R.
AU - Qi, Z.
AU - Wang, J. H.
AU - Cohen, P.
PY - 1995/12
Y1 - 1995/12
N2 - Abstract: The paired helical filament (PHF), which makes up the major fibrous component of the neurofibrillary lesions of Alzheimer's disease, is composed of hyperphosphorylated and abnormally phosphorylated microtubule‐associated protein τ. Previous studies have identified serine and threonine residues phosphorylated in PHF‐τ and have shown that τ can be phosphorylated at several of these sites by proline‐directed protein kinases and cyclic AMP‐dependent protein kinase. Here we have investigated which protein phosphatase activities can dephosphorylate recombinant τ phosphorylated with mitogen‐activated protein kinase, glycogen synthase kinase‐3β, neuronal cdc2‐like kinase, or cyclic AMP‐dependent protein kinase. We show that protein phosphatase 2A is by far the major protein phosphatase activity in brain that dephosphorylates τ phosphorylated in this manner.
AB - Abstract: The paired helical filament (PHF), which makes up the major fibrous component of the neurofibrillary lesions of Alzheimer's disease, is composed of hyperphosphorylated and abnormally phosphorylated microtubule‐associated protein τ. Previous studies have identified serine and threonine residues phosphorylated in PHF‐τ and have shown that τ can be phosphorylated at several of these sites by proline‐directed protein kinases and cyclic AMP‐dependent protein kinase. Here we have investigated which protein phosphatase activities can dephosphorylate recombinant τ phosphorylated with mitogen‐activated protein kinase, glycogen synthase kinase‐3β, neuronal cdc2‐like kinase, or cyclic AMP‐dependent protein kinase. We show that protein phosphatase 2A is by far the major protein phosphatase activity in brain that dephosphorylates τ phosphorylated in this manner.
KW - Dephosphorylation
KW - Paired helical filaments
KW - Protein phosphatase 2A
KW - τ protein
UR - http://www.scopus.com/inward/record.url?scp=0028885494&partnerID=8YFLogxK
U2 - 10.1046/j.1471-4159.1995.65062804.x
DO - 10.1046/j.1471-4159.1995.65062804.x
M3 - Article
C2 - 7595582
AN - SCOPUS:0028885494
SN - 0022-3042
VL - 65
SP - 2804
EP - 2807
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 6
ER -