TY - JOUR
T1 - Protein Phosphatase-2C from Rabbit Skeletal Muscle and Liver
T2 - An Mg2+-Dependent Enzyme
AU - McGowan, Clare H.
AU - Cohen, Philip
PY - 1988
Y1 - 1988
N2 - Protein phosphatase-2C (PP-2C), an Mg2+-dependent enzyme, is one of four protein phosphatase catalytic subunits in the cytoplasm of mammalian cells that are capable of dephosphorylating a variety of regulatory proteins. The enzyme can be purified from skeletal muscle and other mammalian tissues. This chapter describes the procedures for the purification. PP-2C is most conveniently assayed by its ability to dephosphorylate casein, although other substrates, such as phosphorylase kinase and myosin P-light chain, can also be used. PP-2C cannot be assayed reliably at early stages of the preparation, because it represents only a very small proportion of the phosphatase activity toward casein and most other substrates. The major casein phosphatase activity in tissue extracts is protein phosphatase-2A (PP-2A). The purification procedure should be applicable to the isolation of PP-2C from all mammalian tissues.
AB - Protein phosphatase-2C (PP-2C), an Mg2+-dependent enzyme, is one of four protein phosphatase catalytic subunits in the cytoplasm of mammalian cells that are capable of dephosphorylating a variety of regulatory proteins. The enzyme can be purified from skeletal muscle and other mammalian tissues. This chapter describes the procedures for the purification. PP-2C is most conveniently assayed by its ability to dephosphorylate casein, although other substrates, such as phosphorylase kinase and myosin P-light chain, can also be used. PP-2C cannot be assayed reliably at early stages of the preparation, because it represents only a very small proportion of the phosphatase activity toward casein and most other substrates. The major casein phosphatase activity in tissue extracts is protein phosphatase-2A (PP-2A). The purification procedure should be applicable to the isolation of PP-2C from all mammalian tissues.
UR - http://www.scopus.com/inward/record.url?scp=0023774747&partnerID=8YFLogxK
U2 - 10.1016/0076-6879(88)59041-9
DO - 10.1016/0076-6879(88)59041-9
M3 - Article
C2 - 2842606
AN - SCOPUS:0023774747
VL - 159
SP - 416
EP - 426
JO - Methods in Enzymology
JF - Methods in Enzymology
SN - 0076-6879
IS - C
ER -