Protein Phosphatase-2C from Rabbit Skeletal Muscle and Liver: An Mg²⁺-Dependent Enzyme

TY - JOUR

T1 - Protein Phosphatase-2C from Rabbit Skeletal Muscle and Liver

T2 - An Mg2+-Dependent Enzyme

AU - McGowan, Clare H.

AU - Cohen, Philip

PY - 1988

Y1 - 1988

N2 - Protein phosphatase-2C (PP-2C), an Mg2+-dependent enzyme, is one of four protein phosphatase catalytic subunits in the cytoplasm of mammalian cells that are capable of dephosphorylating a variety of regulatory proteins. The enzyme can be purified from skeletal muscle and other mammalian tissues. This chapter describes the procedures for the purification. PP-2C is most conveniently assayed by its ability to dephosphorylate casein, although other substrates, such as phosphorylase kinase and myosin P-light chain, can also be used. PP-2C cannot be assayed reliably at early stages of the preparation, because it represents only a very small proportion of the phosphatase activity toward casein and most other substrates. The major casein phosphatase activity in tissue extracts is protein phosphatase-2A (PP-2A). The purification procedure should be applicable to the isolation of PP-2C from all mammalian tissues.

AB - Protein phosphatase-2C (PP-2C), an Mg2+-dependent enzyme, is one of four protein phosphatase catalytic subunits in the cytoplasm of mammalian cells that are capable of dephosphorylating a variety of regulatory proteins. The enzyme can be purified from skeletal muscle and other mammalian tissues. This chapter describes the procedures for the purification. PP-2C is most conveniently assayed by its ability to dephosphorylate casein, although other substrates, such as phosphorylase kinase and myosin P-light chain, can also be used. PP-2C cannot be assayed reliably at early stages of the preparation, because it represents only a very small proportion of the phosphatase activity toward casein and most other substrates. The major casein phosphatase activity in tissue extracts is protein phosphatase-2A (PP-2A). The purification procedure should be applicable to the isolation of PP-2C from all mammalian tissues.

UR - https://www.scopus.com/pages/publications/0023774747

U2 - 10.1016/0076-6879(88)59041-9

DO - 10.1016/0076-6879(88)59041-9

M3 - Article

C2 - 2842606

AN - SCOPUS:0023774747

SN - 0076-6879

VL - 159

SP - 416

EP - 426

JO - Methods in Enzymology

JF - Methods in Enzymology

IS - C

ER -