Proteomic analysis of the Arabidopsis nucleolus suggests novel nucleolar functions

Alison F. Pendle, Gillian P. Clark, Reinier Boon, Dominika Lewandowska, Yun Wah Lam, Jens Andersen, Matthias Mann, Angus I. Lamond, John W. S. Brown, Peter J. Shaw

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    Abstract

    The eukaryotic nucleolus is involved in ribosome biogenesis and a wide range of other RNA metabolism and cellular functions. An important step in the functional analysis of the nucleolus is to determine the complement of proteins of this nuclear compartment. Here, we describe the first proteomic analysis of plant (Arabidopsis thaliana) nucleoli, in which we have identified 217 proteins. This allows a direct comparison of the proteomes of an important nuclear structure between two widely divergent species: human and Arabidopsis. The comparison identified many common proteins, plant-specific proteins, proteins of unknown function found in both proteomes, and proteins that were nucleolar in plants but nonnucleolar in human. Seventy-two proteins were expressed as GFP fusions and 87% showed nucleolar or nucleolar-associated localization. In a striking and unexpected finding, we have identified six components of the postsplicing exon-junction complex (EJC) involved in mRNA export and nonsense-mediated decay (NMD)/mRNA surveillance. This association was confirmed by GFP-fusion protein localization. These results raise the possibility that in plants, nucleoli may have additional functions in mRNA export or surveillance.
    Original languageEnglish
    Pages (from-to)260-269
    Number of pages10
    JournalMolecular Biology of the Cell
    Volume16
    Issue number1
    DOIs
    Publication statusPublished - 2005

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    Keywords

    • Arabidopsis
    • Plant science
    • Plant products

    Cite this

    Pendle, A. F., Clark, G. P., Boon, R., Lewandowska, D., Lam, Y. W., Andersen, J., Mann, M., Lamond, A. I., Brown, J. W. S., & Shaw, P. J. (2005). Proteomic analysis of the Arabidopsis nucleolus suggests novel nucleolar functions. Molecular Biology of the Cell, 16(1), 260-269. https://doi.org/10.1091/mbc.E04-09-0791