Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control

Jonathan Bizarro, Christophe Charron, Severine Boulon, Belinda Westman, Berengere Pradet-Balade, Franck Vandermoere, Marie-Eve Chagot, Marie Hallais, Yasmeen Ahmad, Heinrich Leonhardt, Angus Lamond, Xavier Manival, Christiane Branlant, Bruno Charpentier, Celine Verheggen (Lead / Corresponding author), Edouard Bertrand (Lead / Corresponding author)

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    Abstract

    In vitro, assembly of box C/D small nucleolar ribonucleoproteins (snoRNPs) involves the sequential recruitment of core proteins to snoRNAs. In vivo, however, assembly factors are required (NUFIP, BCD1, and the HSP90–R2TP complex), and it is unknown whether a similar sequential scheme applies. In this paper, we describe systematic quantitative stable isotope labeling by amino acids in cell culture proteomic experiments and the crystal structure of the core protein Snu13p/15.5K bound to a fragment of the assembly factor Rsa1p/NUFIP. This revealed several unexpected features: (a) the existence of a protein-only pre-snoRNP complex containing five assembly factors and two core proteins, 15.5K and Nop58; (b) the characterization of ZNHIT3, which is present in the protein-only complex but gets released upon binding to C/D snoRNAs; (c) the dynamics of the R2TP complex, which appears to load/unload RuvBL AAA+ adenosine triphosphatase from pre-snoRNPs; and (d) a potential mechanism for preventing premature activation of snoRNP catalytic activity. These data provide a framework for understanding the assembly of box C/D snoRNPs.
    Original languageEnglish
    Pages (from-to)463-480
    Number of pages18
    JournalJournal of Cell Biology
    Volume207
    Issue number4
    DOIs
    Publication statusPublished - 24 Nov 2014

    Cite this

    Bizarro, J., Charron, C., Boulon, S., Westman, B., Pradet-Balade, B., Vandermoere, F., Chagot, M-E., Hallais, M., Ahmad, Y., Leonhardt, H., Lamond, A., Manival, X., Branlant, C., Charpentier, B., Verheggen, C., & Bertrand, E. (2014). Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control. Journal of Cell Biology, 207(4), 463-480. https://doi.org/10.1083/jcb.201404160