Proteomic and microarray analyses of the Dictyostelium Zak1-GSK-3 signaling pathway reveal a role in early development

Lana Strmecki, Gareth Bloomfield, Tsuyoshi Araki, Emma Dalton, Jason Skelton, Christina Schilde, Adrian Harwood, Jeffrey G. Williams, Al Ivens, Catherine Pears (Lead / Corresponding author)

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

GskA, the Dictyostelium GSK-3 orthologue, is modified and activated by the dual-specificity tyrosine kinase Zak1, and the two kinases form part of a signaling pathway that responds to extracellular cyclic AMP. We identify potential cellular effectors for the two kinases by analyzing the corresponding null mutants. There are proteins and mRNAs that are altered in abundance in only one or the other of the two mutants, indicating that each kinase lias some unique functions. However, proteomic and microarray analyses identified a number of proteins and genes, respectively, that are similarly misregulated in both mutant strains. The positive correlation between the array data and the proteomic data is consistent with the Zak1-GskA signaling pathway's functioning by directly or indirectly regulating gene expression. The discoidin 1 genes are positively regulated by the pathway, while the abundance of the H5 protein is negatively regulated. Two of the targets, H5 and discoidin 1, are well-characterized markers for early development, indicating that the Zak1-GskA pathway plays a role in development earlier than previously observed.

Original languageEnglish
Pages (from-to)245-252
Number of pages8
JournalEukaryotic Cell
Volume6
Issue number2
DOIs
Publication statusPublished - 8 Feb 2007

Fingerprint Dive into the research topics of 'Proteomic and microarray analyses of the Dictyostelium Zak1-GSK-3 signaling pathway reveal a role in early development'. Together they form a unique fingerprint.

  • Cite this

    Strmecki, L., Bloomfield, G., Araki, T., Dalton, E., Skelton, J., Schilde, C., Harwood, A., Williams, J. G., Ivens, A., & Pears, C. (2007). Proteomic and microarray analyses of the Dictyostelium Zak1-GSK-3 signaling pathway reveal a role in early development. Eukaryotic Cell, 6(2), 245-252. https://doi.org/10.1128/EC.00204-06