TY - JOUR
T1 - Proteomics of human aqueous humor
AU - Murthy, Krishna R.
AU - Rajagopalan, Pavithra
AU - Pinto, Sneha M.
AU - Advani, Jayshree
AU - Murthy, Praveen R.
AU - Goel, Renu
AU - Subbannayya, Yashwanth
AU - Balakrishnan, Lavanya
AU - Dash, Mahashweta
AU - Anil, Abhijith K.
AU - Manda, Srikanth S.
AU - Nirujogi, Raja Sekhar
AU - Kelkar, Dhanashree S.
AU - Sathe, Gajanan J.
AU - Dey, Gourav
AU - Chatterjee, Aditi
AU - Gowda, Harsha
AU - Chakravarti, Shukti
AU - Shankar, Subramanian
AU - Sahasrabuddhe, Nandini A.
AU - Nair, Bipin
AU - Somani, Babu Lal
AU - Prasad, T. S.Keshava
AU - Pandey, Akhilesh
N1 - Publisher Copyright:
© 2015, Mary Ann Liebert, Inc.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2015/5
Y1 - 2015/5
N2 - The aqueous humor is a colorless, transparent fluid that fills the anterior chamber of the eye. It plays an important role in maintaining the intraocular pressure and providing nourishment to the lens and cornea. The constitution of the aqueous humor is controlled by the blood-aqueous barrier. Though this ocular fluid has been extensively studied, its role in ocular physiology is still not completely understood. In this study, aqueous humor samples were collected from 250 patients undergoing cataract surgery, subjected to multiple fractionation strategies and analyzed on a Fourier transform LTQ-Orbitrap Velos mass spectrometer. In all, we identified 763 proteins, of which 386 have been identified for the first time in this study. Sorbitol dehydrogenase (SORD), filensin (BFSP1), and phakinin (BFSP2) are some of the proteins that have not been previously reported in the aqueous humor. Gene Ontology analysis revealed 35% of the identified proteins to be extracellular, with a majority of them involved in cell communication and signal transduction. This study comprehensively reports 386 novel proteins that have important potential as biomarker candidates for future research into personalized medicine and diagnostics aimed towards improving visual health.
AB - The aqueous humor is a colorless, transparent fluid that fills the anterior chamber of the eye. It plays an important role in maintaining the intraocular pressure and providing nourishment to the lens and cornea. The constitution of the aqueous humor is controlled by the blood-aqueous barrier. Though this ocular fluid has been extensively studied, its role in ocular physiology is still not completely understood. In this study, aqueous humor samples were collected from 250 patients undergoing cataract surgery, subjected to multiple fractionation strategies and analyzed on a Fourier transform LTQ-Orbitrap Velos mass spectrometer. In all, we identified 763 proteins, of which 386 have been identified for the first time in this study. Sorbitol dehydrogenase (SORD), filensin (BFSP1), and phakinin (BFSP2) are some of the proteins that have not been previously reported in the aqueous humor. Gene Ontology analysis revealed 35% of the identified proteins to be extracellular, with a majority of them involved in cell communication and signal transduction. This study comprehensively reports 386 novel proteins that have important potential as biomarker candidates for future research into personalized medicine and diagnostics aimed towards improving visual health.
UR - http://www.scopus.com/inward/record.url?scp=84929081187&partnerID=8YFLogxK
U2 - 10.1089/omi.2015.0029
DO - 10.1089/omi.2015.0029
M3 - Article
C2 - 25933257
AN - SCOPUS:84929081187
SN - 1536-2310
VL - 19
SP - 283
EP - 293
JO - OMICS: A Journal of Integrative Biology (OMICS)
JF - OMICS: A Journal of Integrative Biology (OMICS)
IS - 5
ER -