Protocol for a placenta-on-a-chip model using trophoblasts differentiated from human induced pluripotent stem cells

Agathe Lermant, Gwenaëlle Rabussier, Lindsey Davidson, Henriëtte L. Lanz, Colin E. Murdoch (Lead / Corresponding author)

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Abstract

Organ-on-a-chip technology incorporating stem cell techniques represents a promising strategy to improve modeling of human organs. Here, we present a protocol for generating a standardized 3D placenta-on-a-chip model using trophoblast derived from human induced pluripotent stem cells (hiPSCs). We describe steps for seeding hiPSCs into multi-chip OrganoPlate devices and on-chip differentiation into trophoblasts against an extracellular matrix under perfused conditions. We then detail procedures for conducting a functional barrier integrity assay, immunostaining, and collecting protein or RNA for molecular analysis. For complete details on the use and execution of this protocol, please refer to Lermant et al. (2023).

Original languageEnglish
Article number102879
Number of pages21
JournalSTAR Protocols
Volume5
Issue number1
Early online date14 Feb 2024
DOIs
Publication statusPublished - 15 Mar 2024

Keywords

  • Biotechnology and bioengineering
  • Organoids
  • Systems biology

ASJC Scopus subject areas

  • General Immunology and Microbiology
  • General Biochemistry,Genetics and Molecular Biology
  • General Neuroscience

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