@inbook{107c3fc1b96b407f94d1645000e29a00,
title = "Protoplast Transformation of Phytophthora spp.",
abstract = "At the core of assays to understand the role(s) of specific genes is the ability to stably transfer genes into Phytophthora through transformation. A key method for achieving this has been based on polyethylene glycol (PEG)/CaCl2 transformation of protoplasts, but efficiency has often been low. Improving transformation efficiency is necessary for many applications, such as gene knockouts. Here we describe improvements through successive rounds of “mock” transformation, leading to improved efficiency in Phytophthora infestans and other species.",
keywords = "Antibiotic selection, Oomycete, Phytophthora, Protoplasts, Transformation",
author = "Welsh, \{Lydia R.J.\} and Whisson, \{Stephen C.\}",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.",
year = "2024",
month = dec,
day = "28",
doi = "10.1007/978-1-0716-4330-3\_3",
language = "English",
isbn = "9781071643297",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "35--47",
editor = "Gerth, \{Monica L.\} and Bradshaw, \{Rosie E.\}",
booktitle = "Methods in Molecular Biology",
address = "United States",
}