PTEN protein phosphatase activity correlates with control of gene expression and invasion, a tumor-suppressing phenotype, but not with AKT activity

Priyanka Tibarewal, Georgios Zilidis, Laura Spinelli, Nick Schurch, Helene Maccario, Alexander Gray, Nevin M. Perera, Lindsay Davidson, Geoffrey J Barton, Nick R. Leslie

    Research output: Contribution to journalArticle

    67 Citations (Scopus)

    Abstract

    The tumor suppressor phosphatase and tensin homolog deleted on chromosome 10 (PTEN) has a well-characterized lipid phosphatase activity and a poorly characterized protein phosphatase activity. We show that both activities are required for PTEN to inhibit cellular invasion and to mediate most of its largest effects on gene expression. PTEN appears to dephosphorylate itself at threonine 366, and mutation of this site makes lipid phosphatase activity sufficient for PTEN to inhibit invasion. We propose that the dominant role for PTEN's protein phosphatase activity is autodephosphorylation-mediated regulation of its lipid phosphatase activity. Because PTEN's regulation of invasion and these changes in gene expression required lipid phosphatase activity, but did not correlate with the total cellular abundance of its phosphatidylinositol 3,4,5-trisphosphate (PIP3) lipid substrate or AKT activity, we propose that localized PIP3 signaling may play a role in those PTEN-mediated processes that depend on both its protein and lipid phosphatase activities. Finally, we identified a tumor-derived PTEN mutant selectively lacking protein phosphatase activity, indicating that in some circumstances the regulation of invasion and not that of AKT can correlate with PTEN-mediated tumor suppression.
    Original languageEnglish
    Pages (from-to)ra18
    JournalScience Signaling
    Volume5
    Issue number213
    DOIs
    Publication statusPublished - 2012

    Fingerprint

    PTEN Phosphohydrolase
    Phosphoric Monoester Hydrolases
    Gene expression
    Tumors
    Phenotype
    Lipids
    Gene Expression
    Phosphoprotein Phosphatases
    Neoplasms
    Chromosomes, Human, Pair 10
    Threonine
    Chromosomes
    Mutation
    Substrates

    Cite this

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    title = "PTEN protein phosphatase activity correlates with control of gene expression and invasion, a tumor-suppressing phenotype, but not with AKT activity",
    abstract = "The tumor suppressor phosphatase and tensin homolog deleted on chromosome 10 (PTEN) has a well-characterized lipid phosphatase activity and a poorly characterized protein phosphatase activity. We show that both activities are required for PTEN to inhibit cellular invasion and to mediate most of its largest effects on gene expression. PTEN appears to dephosphorylate itself at threonine 366, and mutation of this site makes lipid phosphatase activity sufficient for PTEN to inhibit invasion. We propose that the dominant role for PTEN's protein phosphatase activity is autodephosphorylation-mediated regulation of its lipid phosphatase activity. Because PTEN's regulation of invasion and these changes in gene expression required lipid phosphatase activity, but did not correlate with the total cellular abundance of its phosphatidylinositol 3,4,5-trisphosphate (PIP3) lipid substrate or AKT activity, we propose that localized PIP3 signaling may play a role in those PTEN-mediated processes that depend on both its protein and lipid phosphatase activities. Finally, we identified a tumor-derived PTEN mutant selectively lacking protein phosphatase activity, indicating that in some circumstances the regulation of invasion and not that of AKT can correlate with PTEN-mediated tumor suppression.",
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    PTEN protein phosphatase activity correlates with control of gene expression and invasion, a tumor-suppressing phenotype, but not with AKT activity. / Tibarewal, Priyanka; Zilidis, Georgios; Spinelli, Laura; Schurch, Nick; Maccario, Helene; Gray, Alexander; Perera, Nevin M.; Davidson, Lindsay; Barton, Geoffrey J; Leslie, Nick R.

    In: Science Signaling, Vol. 5, No. 213, 2012, p. ra18.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - PTEN protein phosphatase activity correlates with control of gene expression and invasion, a tumor-suppressing phenotype, but not with AKT activity

    AU - Tibarewal, Priyanka

    AU - Zilidis, Georgios

    AU - Spinelli, Laura

    AU - Schurch, Nick

    AU - Maccario, Helene

    AU - Gray, Alexander

    AU - Perera, Nevin M.

    AU - Davidson, Lindsay

    AU - Barton, Geoffrey J

    AU - Leslie, Nick R.

    PY - 2012

    Y1 - 2012

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    AB - The tumor suppressor phosphatase and tensin homolog deleted on chromosome 10 (PTEN) has a well-characterized lipid phosphatase activity and a poorly characterized protein phosphatase activity. We show that both activities are required for PTEN to inhibit cellular invasion and to mediate most of its largest effects on gene expression. PTEN appears to dephosphorylate itself at threonine 366, and mutation of this site makes lipid phosphatase activity sufficient for PTEN to inhibit invasion. We propose that the dominant role for PTEN's protein phosphatase activity is autodephosphorylation-mediated regulation of its lipid phosphatase activity. Because PTEN's regulation of invasion and these changes in gene expression required lipid phosphatase activity, but did not correlate with the total cellular abundance of its phosphatidylinositol 3,4,5-trisphosphate (PIP3) lipid substrate or AKT activity, we propose that localized PIP3 signaling may play a role in those PTEN-mediated processes that depend on both its protein and lipid phosphatase activities. Finally, we identified a tumor-derived PTEN mutant selectively lacking protein phosphatase activity, indicating that in some circumstances the regulation of invasion and not that of AKT can correlate with PTEN-mediated tumor suppression.

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