Purification and characterisation of the insulin‐stimulated protein kinase from rabbit skeletal muscle; close similarity to S6 kinase II

Alain Lavoinne, Eleanor Erikson, James L. Maller, Daniel J. Price, Joseph Avruch, Philip COHEN

    Research output: Contribution to journalArticle

    71 Citations (Scopus)

    Abstract

    The insulin‐stimulated protein kinase (ISPK) was purified over 50000‐fold from extracts of rabbit skeletal muscle by a procedure involving chromatography on phosphocellulose, fractionation with ammonium sulphate, and further chromatography on DEAE‐cellulose, phenyl‐Superose, Mono S and Mono Q. About 10 μg enzyme was isolated from 800 g muscle (one rabbit) in four days with an overall recovery of 5%. The purified enzyme showed a single protein‐staining band of apparent molecular mass 91 kDa when analysed by SDS/polyacrylamide gel electrophoresis. The ISPK comigrated during SDS/polyacrylamide gel electrophoresis with the enzyme S6 kinase II from Xenopus eggs, and was recognised in immunoblotting and immunoprecipitation experiments by antibodies raised against S6 kinase II. The substrate specificities of ISPK and S6 kinase II were also very similar and like S6 kinase II, ISPK that had been inactivated by protein phosphatase 2A could be reactivated by incubation with mitogen‐activated protein kinase and MgATP. ISPK was distinct from an insulin‐stimulated 70‐kDa S6 kinase from rat liver in both substrate specificity and immunological cross reactivity. It is concluded that ISPK is closely related in structure to S6 kinase II and may be a mammalian equivalent of this enzyme. The possibility that ISPK is involved in mediating a number of the actions of insulin is discussed.

    Original languageEnglish
    Pages (from-to)723-728
    Number of pages6
    JournalEuropean Journal of Biochemistry
    Volume199
    Issue number3
    DOIs
    Publication statusPublished - Aug 1991

    Fingerprint

    Ribosomal Protein S6 Kinases
    Protein Kinases
    Purification
    Muscle
    Skeletal Muscle
    Rabbits
    Enzymes
    Substrate Specificity
    Chromatography
    Electrophoresis
    Polyacrylamide Gel Electrophoresis
    Protein Phosphatase 2
    Ammonium Sulfate
    Molecular mass
    Substrates
    Fractionation
    Xenopus
    Immunoprecipitation
    Immunoblotting
    Liver

    Cite this

    Lavoinne, Alain ; Erikson, Eleanor ; Maller, James L. ; Price, Daniel J. ; Avruch, Joseph ; COHEN, Philip. / Purification and characterisation of the insulin‐stimulated protein kinase from rabbit skeletal muscle; close similarity to S6 kinase II. In: European Journal of Biochemistry. 1991 ; Vol. 199, No. 3. pp. 723-728.
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    Purification and characterisation of the insulin‐stimulated protein kinase from rabbit skeletal muscle; close similarity to S6 kinase II. / Lavoinne, Alain; Erikson, Eleanor; Maller, James L.; Price, Daniel J.; Avruch, Joseph; COHEN, Philip.

    In: European Journal of Biochemistry, Vol. 199, No. 3, 08.1991, p. 723-728.

    Research output: Contribution to journalArticle

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