Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase

TY - JOUR

T1 - Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase

AU - Seetharamappa, Jaldappagari

AU - Oke, Muse

AU - Liu, Huanting

AU - McMahon, Stephen A.

AU - Johnson, Kenneth A.

AU - Carter, Lester

AU - Dorward, Mark

AU - Zawadzki, Michal

AU - Overton, Ian M.

AU - Van Niekirk, C. A.Johannes

AU - Graham, Shirley

AU - Botting, Catherine H.

AU - Taylor, Garry L.

AU - White, Malcolm F.

AU - Barton, Geoffrey J.

AU - Coote, Peter J.

AU - Naismith, James H.

PY - 2007/5/5

Y1 - 2007/5/5

N2 - Sar2676, a pantothenate synthetase with a molecular weight of 31 419 Da from methicillin-resistant Staphylococcus aureus, has been expressed, purified and crystallized at 293 K. The protein crystallizes in a primitive triclinic lattice, with unit-cell parameters a = 45.3, b = 60.5, c = 117.6 Å, α = 87.2, β = 81.2, γ = 68.4°. A complete data set has been collected to 2.3 Å resolution at the ESRF. Consideration of the likely solvent content suggested the asymmetric unit to contain four molecules. This has been confirmed by molecular-replacement phasing calculations, which give a solution with four monomers using a monomer of pantothenate synthetase from Escherichia coli (PDB code 1iho), which is 41% identical to Sar2676, as a search model.

AB - Sar2676, a pantothenate synthetase with a molecular weight of 31 419 Da from methicillin-resistant Staphylococcus aureus, has been expressed, purified and crystallized at 293 K. The protein crystallizes in a primitive triclinic lattice, with unit-cell parameters a = 45.3, b = 60.5, c = 117.6 Å, α = 87.2, β = 81.2, γ = 68.4°. A complete data set has been collected to 2.3 Å resolution at the ESRF. Consideration of the likely solvent content suggested the asymmetric unit to contain four molecules. This has been confirmed by molecular-replacement phasing calculations, which give a solution with four monomers using a monomer of pantothenate synthetase from Escherichia coli (PDB code 1iho), which is 41% identical to Sar2676, as a search model.

KW - Methicillin-resistant Staphylococcus aureus

KW - Pantothenate synthetase

KW - Sar2676

UR - https://www.scopus.com/pages/publications/34249947297

U2 - 10.1107/S1744309107020362

DO - 10.1107/S1744309107020362

M3 - Article

C2 - 17554169

AN - SCOPUS:34249947297

SN - 1744-3091

VL - 63

SP - 488

EP - 491

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

IS - 6

ER -