Purification, crystallization and preliminary X-ray diffraction data of UDP-galactopyranose mutase from Aspergillus fumigatus

G. A. Penman, D. E. A. Lockhart, A. Ferenbach, D. M. F. Van Aalten

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Aspergillus fumigatus UDP-galactopyranose mutase (AfUGM) is a potential drug target involved in the synthesis of the cell wall of this fungal pathogen. AfUGM was recombinantly produced in Escherichia coli, purified and crystallized by the sitting-drop method, producing orthorhombic crystals that diffracted to a resolution of 3.25 Å. The crystals contained four molecules per asymmetric unit and belonged to space group P2 2 2 , with unit-cell parameters a = 127.72, b = 134.30, c = 173.84 Å. Incorporation of selenomethionine was achieved, but the resulting crystals did not allow solution of the phase problem.
    Original languageEnglish
    Pages (from-to)705-708
    Number of pages4
    JournalActa Crystallographica F-Structural Biology and Crystallization Communications
    Volume68
    Issue number6
    DOIs
    Publication statusPublished - 2012

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