Purification of human hepatic glutathione S-transferases and the development of a radioimmunoassay for their measurement in plasma

J D Hayes, D Gilligan, B J Chapman, G J Beckett

    Research output: Contribution to journalArticle

    43 Citations (Scopus)

    Abstract

    A purification scheme is described for six human hepatic glutathione S-transferases from a single liver. Five of the transferases comprised Ya monomers and had a molecular mass of 44 000. The remaining enzyme comprised Yb monomers and had a molecular mass of 47 000. Data are presented demonstrating that there are at least two distinct Ya monomers. A radioimmunoassay has been developed that has sufficient precision and sensitivity to allow direct measurement of glutathione S-transferase concentrations in unextracted plasma. A comparison of aminotransferase and glutathione S-transferase levels, in three patients who had taken a paracetamol overdose, indicated that glutathione S-transferase measurements provided a far more sensitive index of hepatocellular integrity than the more conventional aminotransferase measurements.

    Original languageEnglish
    Pages (from-to)107-21
    Number of pages15
    JournalClinica Chimica Acta
    Volume134
    Issue number1-2
    Publication statusPublished - 31 Oct 1983

    Fingerprint

    Glutathione Transferase
    Radioimmunoassay
    Purification
    Plasmas
    Monomers
    Liver
    Molecular mass
    Transaminases
    Acetaminophen
    Transferases
    Enzymes

    Keywords

    • Alanine Transaminase/blood
    • Aspartate Aminotransferases/blood
    • Chromatography, Affinity/methods
    • Cross Reactions
    • Glutathione Transferase/blood
    • Humans
    • Liver/enzymology
    • Male
    • Middle Aged
    • Molecular Weight
    • Radioimmunoassay/methods

    Cite this

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    title = "Purification of human hepatic glutathione S-transferases and the development of a radioimmunoassay for their measurement in plasma",
    abstract = "A purification scheme is described for six human hepatic glutathione S-transferases from a single liver. Five of the transferases comprised Ya monomers and had a molecular mass of 44 000. The remaining enzyme comprised Yb monomers and had a molecular mass of 47 000. Data are presented demonstrating that there are at least two distinct Ya monomers. A radioimmunoassay has been developed that has sufficient precision and sensitivity to allow direct measurement of glutathione S-transferase concentrations in unextracted plasma. A comparison of aminotransferase and glutathione S-transferase levels, in three patients who had taken a paracetamol overdose, indicated that glutathione S-transferase measurements provided a far more sensitive index of hepatocellular integrity than the more conventional aminotransferase measurements.",
    keywords = "Alanine Transaminase/blood, Aspartate Aminotransferases/blood, Chromatography, Affinity/methods, Cross Reactions, Glutathione Transferase/blood, Humans, Liver/enzymology, Male, Middle Aged, Molecular Weight, Radioimmunoassay/methods",
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    Purification of human hepatic glutathione S-transferases and the development of a radioimmunoassay for their measurement in plasma. / Hayes, J D; Gilligan, D; Chapman, B J; Beckett, G J.

    In: Clinica Chimica Acta, Vol. 134, No. 1-2, 31.10.1983, p. 107-21.

    Research output: Contribution to journalArticle

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    T1 - Purification of human hepatic glutathione S-transferases and the development of a radioimmunoassay for their measurement in plasma

    AU - Hayes, J D

    AU - Gilligan, D

    AU - Chapman, B J

    AU - Beckett, G J

    PY - 1983/10/31

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    N2 - A purification scheme is described for six human hepatic glutathione S-transferases from a single liver. Five of the transferases comprised Ya monomers and had a molecular mass of 44 000. The remaining enzyme comprised Yb monomers and had a molecular mass of 47 000. Data are presented demonstrating that there are at least two distinct Ya monomers. A radioimmunoassay has been developed that has sufficient precision and sensitivity to allow direct measurement of glutathione S-transferase concentrations in unextracted plasma. A comparison of aminotransferase and glutathione S-transferase levels, in three patients who had taken a paracetamol overdose, indicated that glutathione S-transferase measurements provided a far more sensitive index of hepatocellular integrity than the more conventional aminotransferase measurements.

    AB - A purification scheme is described for six human hepatic glutathione S-transferases from a single liver. Five of the transferases comprised Ya monomers and had a molecular mass of 44 000. The remaining enzyme comprised Yb monomers and had a molecular mass of 47 000. Data are presented demonstrating that there are at least two distinct Ya monomers. A radioimmunoassay has been developed that has sufficient precision and sensitivity to allow direct measurement of glutathione S-transferase concentrations in unextracted plasma. A comparison of aminotransferase and glutathione S-transferase levels, in three patients who had taken a paracetamol overdose, indicated that glutathione S-transferase measurements provided a far more sensitive index of hepatocellular integrity than the more conventional aminotransferase measurements.

    KW - Alanine Transaminase/blood

    KW - Aspartate Aminotransferases/blood

    KW - Chromatography, Affinity/methods

    KW - Cross Reactions

    KW - Glutathione Transferase/blood

    KW - Humans

    KW - Liver/enzymology

    KW - Male

    KW - Middle Aged

    KW - Molecular Weight

    KW - Radioimmunoassay/methods

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    JO - Clinica Chimica Acta

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