Purification of human hepatic glutathione S-transferases and the development of a radioimmunoassay for their measurement in plasma

J D Hayes; D Gilligan; B J Chapman; G J Beckett

Purification of human hepatic glutathione S-transferases and the development of a radioimmunoassay for their measurement in plasma

J D Hayes
, D Gilligan
, B J Chapman
, G J Beckett

Research output: Contribution to journal › Article › peer-review

Abstract

A purification scheme is described for six human hepatic glutathione S-transferases from a single liver. Five of the transferases comprised Ya monomers and had a molecular mass of 44 000. The remaining enzyme comprised Yb monomers and had a molecular mass of 47 000. Data are presented demonstrating that there are at least two distinct Ya monomers. A radioimmunoassay has been developed that has sufficient precision and sensitivity to allow direct measurement of glutathione S-transferase concentrations in unextracted plasma. A comparison of aminotransferase and glutathione S-transferase levels, in three patients who had taken a paracetamol overdose, indicated that glutathione S-transferase measurements provided a far more sensitive index of hepatocellular integrity than the more conventional aminotransferase measurements.

Original language	English
Pages (from-to)	107-21
Number of pages	15
Journal	Clinica Chimica Acta
Volume	134
Issue number	1-2
Publication status	Published - 31 Oct 1983

Keywords

Alanine Transaminase/blood
Aspartate Aminotransferases/blood
Chromatography, Affinity/methods
Cross Reactions
Glutathione Transferase/blood
Humans
Liver/enzymology
Male
Middle Aged
Molecular Weight
Radioimmunoassay/methods

Cite this

@article{c95a197f9f3641c2a869cd337af8c47b,

title = "Purification of human hepatic glutathione S-transferases and the development of a radioimmunoassay for their measurement in plasma",

abstract = "A purification scheme is described for six human hepatic glutathione S-transferases from a single liver. Five of the transferases comprised Ya monomers and had a molecular mass of 44 000. The remaining enzyme comprised Yb monomers and had a molecular mass of 47 000. Data are presented demonstrating that there are at least two distinct Ya monomers. A radioimmunoassay has been developed that has sufficient precision and sensitivity to allow direct measurement of glutathione S-transferase concentrations in unextracted plasma. A comparison of aminotransferase and glutathione S-transferase levels, in three patients who had taken a paracetamol overdose, indicated that glutathione S-transferase measurements provided a far more sensitive index of hepatocellular integrity than the more conventional aminotransferase measurements.",

keywords = "Alanine Transaminase/blood, Aspartate Aminotransferases/blood, Chromatography, Affinity/methods, Cross Reactions, Glutathione Transferase/blood, Humans, Liver/enzymology, Male, Middle Aged, Molecular Weight, Radioimmunoassay/methods",

author = "Hayes, \{J D\} and D Gilligan and Chapman, \{B J\} and Beckett, \{G J\}",

year = "1983",

month = oct,

day = "31",

language = "English",

volume = "134",

pages = "107--21",

journal = "Clinica Chimica Acta",

issn = "0009-8981",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Purification of human hepatic glutathione S-transferases and the development of a radioimmunoassay for their measurement in plasma

AU - Hayes, J D

AU - Gilligan, D

AU - Chapman, B J

AU - Beckett, G J

PY - 1983/10/31

Y1 - 1983/10/31

N2 - A purification scheme is described for six human hepatic glutathione S-transferases from a single liver. Five of the transferases comprised Ya monomers and had a molecular mass of 44 000. The remaining enzyme comprised Yb monomers and had a molecular mass of 47 000. Data are presented demonstrating that there are at least two distinct Ya monomers. A radioimmunoassay has been developed that has sufficient precision and sensitivity to allow direct measurement of glutathione S-transferase concentrations in unextracted plasma. A comparison of aminotransferase and glutathione S-transferase levels, in three patients who had taken a paracetamol overdose, indicated that glutathione S-transferase measurements provided a far more sensitive index of hepatocellular integrity than the more conventional aminotransferase measurements.

AB - A purification scheme is described for six human hepatic glutathione S-transferases from a single liver. Five of the transferases comprised Ya monomers and had a molecular mass of 44 000. The remaining enzyme comprised Yb monomers and had a molecular mass of 47 000. Data are presented demonstrating that there are at least two distinct Ya monomers. A radioimmunoassay has been developed that has sufficient precision and sensitivity to allow direct measurement of glutathione S-transferase concentrations in unextracted plasma. A comparison of aminotransferase and glutathione S-transferase levels, in three patients who had taken a paracetamol overdose, indicated that glutathione S-transferase measurements provided a far more sensitive index of hepatocellular integrity than the more conventional aminotransferase measurements.

KW - Alanine Transaminase/blood

KW - Aspartate Aminotransferases/blood

KW - Chromatography, Affinity/methods

KW - Cross Reactions

KW - Glutathione Transferase/blood

KW - Humans

KW - Liver/enzymology

KW - Male

KW - Middle Aged

KW - Molecular Weight

KW - Radioimmunoassay/methods

UR - https://www.ncbi.nlm.nih.gov/pubmed/6652905

M3 - Article

C2 - 6652905

SN - 0009-8981

VL - 134

SP - 107

EP - 121

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

IS - 1-2

ER -

Purification of human hepatic glutathione S-transferases and the development of a radioimmunoassay for their measurement in plasma

Abstract

Keywords

Other files and links

Fingerprint

Cite this