Qualitative and Quantitative Analysis of Spinach Extracts: A Modular, Inexpensive, and Bench-Friendly Protocol

TY - JOUR

T1 - Qualitative and Quantitative Analysis of Spinach Extracts

T2 - A Modular, Inexpensive, and Bench-Friendly Protocol

AU - D'Ascenzio, Melissa

AU - Black, Alan

AU - Forrest, Bethan

AU - Pokora, John

N1 - Publisher Copyright: © 2025 The Authors. Published by American Chemical Society and Division of Chemical Education, Inc.

PY - 2025/12/9

Y1 - 2025/12/9

N2 - The chromatographic separation of plant pigments is one of the most popular and visually appealing experiments encountered by young scientists when they are introduced to the principles of analytical chemistry. As such, it has found widespread application in schools, public engagement activities, and undergraduate laboratories. However, most of the methods used in these contexts suffer from a range of limitations, including the difficulty of separating and isolating individual pigments when using paper-based or thin layer chromatography techniques or the need for specialized spaces in order to contain volatile, harmful solvents and dry silica powder. This new modular laboratory protocol has been developed following green chemistry principles and employs a series of inexpensive, bench-friendly methods. The protocol allows for the separation and quantitation of chlorophyll pigments in plant extracts through the use of prepacked, inexpensive reversed-phase chromatography columns and UV-vis spectrophotometry. A synthetic component is added to the more traditional separation experiments through the development of the bench-friendly synthesis of coloring agent E141, a chlorophyll-derived food dye. The protocol embeds analytical and mathematical components that encourage students to engage in problem solving activities and mathematical modeling early on in their studies.

AB - The chromatographic separation of plant pigments is one of the most popular and visually appealing experiments encountered by young scientists when they are introduced to the principles of analytical chemistry. As such, it has found widespread application in schools, public engagement activities, and undergraduate laboratories. However, most of the methods used in these contexts suffer from a range of limitations, including the difficulty of separating and isolating individual pigments when using paper-based or thin layer chromatography techniques or the need for specialized spaces in order to contain volatile, harmful solvents and dry silica powder. This new modular laboratory protocol has been developed following green chemistry principles and employs a series of inexpensive, bench-friendly methods. The protocol allows for the separation and quantitation of chlorophyll pigments in plant extracts through the use of prepacked, inexpensive reversed-phase chromatography columns and UV-vis spectrophotometry. A synthetic component is added to the more traditional separation experiments through the development of the bench-friendly synthesis of coloring agent E141, a chlorophyll-derived food dye. The protocol embeds analytical and mathematical components that encourage students to engage in problem solving activities and mathematical modeling early on in their studies.

KW - Analytical Chemistry

KW - Aqueous Solution Chemistry

KW - Bioanalytical Chemistry

KW - Bioorganic Chemistry

KW - Chemometrics

KW - Chromatography

KW - Dyes

KW - First-Year Undergraduate

KW - Green Chemistry

KW - Hands-On Learning

KW - Laboratory Instructions

KW - Mathematics

KW - Pigments

KW - Plant Chemistry

KW - Qualitative and Quantitative Analysis

KW - UV−Vis Spectroscopy

UR - https://www.scopus.com/pages/publications/105024541152

U2 - 10.1021/acs.jchemed.5c00920

DO - 10.1021/acs.jchemed.5c00920

M3 - Article

SN - 0021-9584

VL - 102

SP - 5299

EP - 5308

JO - Journal of Chemical Education

JF - Journal of Chemical Education

IS - 12

ER -