QuaNCAT: Quantitating proteome dynamics in primary cells

Andrew J.M. Howden, Vincent Geoghegan, Kristin Katsch, Georgios Efstathiou, Bhaskar Bhushan, Omar Boutureira, Benjamin Thomas, David C. Trudgian, Benedikt M. Kessler, Daniela C. Dieterich, Benjamin G. Davis, Oreste Acuto

    Research output: Contribution to journalArticlepeer-review

    96 Citations (Scopus)

    Abstract

    Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activation stimuli.
    Original languageEnglish
    Pages (from-to)343-346
    Number of pages4
    JournalNature Methods
    Volume10
    Issue number4
    DOIs
    Publication statusPublished - 2013

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