QuaNCAT: Quantitating proteome dynamics in primary cells

Andrew J.M. Howden; Vincent Geoghegan; Kristin Katsch; Georgios Efstathiou; Bhaskar Bhushan; Omar Boutureira; Benjamin Thomas; David C. Trudgian; Benedikt M. Kessler; Daniela C. Dieterich; Benjamin G. Davis; Oreste Acuto

doi:10.1038/nmeth.2401

QuaNCAT: Quantitating proteome dynamics in primary cells

Andrew J.M. Howden, Vincent Geoghegan, Kristin Katsch, Georgios Efstathiou, Bhaskar Bhushan, Omar Boutureira, Benjamin Thomas, David C. Trudgian, Benedikt M. Kessler, Daniela C. Dieterich, Benjamin G. Davis, Oreste Acuto

Research output: Contribution to journal › Article › peer-review

139 Citations (Scopus)

Abstract

Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activation stimuli.

Original language	English
Pages (from-to)	343-346
Number of pages	4
Journal	Nature Methods
Volume	10
Issue number	4
DOIs	https://doi.org/10.1038/nmeth.2401
Publication status	Published - 2013

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10.1038/nmeth.2401

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abstract = "Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activation stimuli.",

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AU - Geoghegan, Vincent

AU - Katsch, Kristin

AU - Efstathiou, Georgios

AU - Bhushan, Bhaskar

AU - Boutureira, Omar

AU - Thomas, Benjamin

AU - Trudgian, David C.

AU - Kessler, Benedikt M.

AU - Dieterich, Daniela C.

AU - Davis, Benjamin G.

AU - Acuto, Oreste

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QuaNCAT: Quantitating proteome dynamics in primary cells

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