QuaNCAT: Quantitating proteome dynamics in primary cells

TY - JOUR

T1 - QuaNCAT

T2 - Quantitating proteome dynamics in primary cells

AU - Howden, Andrew J.M.

AU - Geoghegan, Vincent

AU - Katsch, Kristin

AU - Efstathiou, Georgios

AU - Bhushan, Bhaskar

AU - Boutureira, Omar

AU - Thomas, Benjamin

AU - Trudgian, David C.

AU - Kessler, Benedikt M.

AU - Dieterich, Daniela C.

AU - Davis, Benjamin G.

AU - Acuto, Oreste

N1 - Copyright 2013 Elsevier B.V., All rights reserved.

PY - 2013

Y1 - 2013

N2 - Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activation stimuli.

AB - Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activation stimuli.

UR - https://www.scopus.com/pages/publications/84875755186

U2 - 10.1038/nmeth.2401

DO - 10.1038/nmeth.2401

M3 - Article

C2 - 23474466

AN - SCOPUS:84875755186

SN - 1548-7091

VL - 10

SP - 343

EP - 346

JO - Nature Methods

JF - Nature Methods

IS - 4

ER -