Quantification of cytochrome P450 reductase gene expression in human tissues

Elizabeth A. Shephard; Colin N.A. Palmer; H. J. Segall; Ian R. Phillips

doi:10.1016/0003-9861(92)90152-M

Quantification of cytochrome P450 reductase gene expression in human tissues

Elizabeth A. Shephard (Lead / Corresponding author), Colin N.A. Palmer, H. J. Segall, Ian R. Phillips

Population Health and Genomics

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

We have isolated and sequenced cDNA clones that code for a variant of human cytochrome P450 reductase. An RNase protection assay was used to quantify the corresponding mRNA in adult and fetal tissues. The results demonstrate that, in the samples analyzed, the cytochrome P450 reductase gene displays very little interindividual variation in its expression in adult liver and is subject to little developmental or tissue-specific regulation.

Original language	English
Pages (from-to)	168-172
Number of pages	5
Journal	Archives of Biochemistry and Biophysics
Volume	294
Issue number	1
DOIs	https://doi.org/10.1016/0003-9861(92)90152-M
Publication status	Published - Apr 1992

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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10.1016/0003-9861(92)90152-M

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title = "Quantification of cytochrome P450 reductase gene expression in human tissues",

abstract = "We have isolated and sequenced cDNA clones that code for a variant of human cytochrome P450 reductase. An RNase protection assay was used to quantify the corresponding mRNA in adult and fetal tissues. The results demonstrate that, in the samples analyzed, the cytochrome P450 reductase gene displays very little interindividual variation in its expression in adult liver and is subject to little developmental or tissue-specific regulation.",

author = "Shephard, {Elizabeth A.} and Palmer, {Colin N.A.} and Segall, {H. J.} and Phillips, {Ian R.}",

year = "1992",

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AU - Shephard, Elizabeth A.

AU - Palmer, Colin N.A.

AU - Segall, H. J.

AU - Phillips, Ian R.

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Y1 - 1992/4

N2 - We have isolated and sequenced cDNA clones that code for a variant of human cytochrome P450 reductase. An RNase protection assay was used to quantify the corresponding mRNA in adult and fetal tissues. The results demonstrate that, in the samples analyzed, the cytochrome P450 reductase gene displays very little interindividual variation in its expression in adult liver and is subject to little developmental or tissue-specific regulation.

AB - We have isolated and sequenced cDNA clones that code for a variant of human cytochrome P450 reductase. An RNase protection assay was used to quantify the corresponding mRNA in adult and fetal tissues. The results demonstrate that, in the samples analyzed, the cytochrome P450 reductase gene displays very little interindividual variation in its expression in adult liver and is subject to little developmental or tissue-specific regulation.

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U2 - 10.1016/0003-9861(92)90152-M

DO - 10.1016/0003-9861(92)90152-M

M3 - Article

C2 - 1550342

AN - SCOPUS:0026572114

SN - 0003-9861

VL - 294

SP - 168

EP - 172

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

IS - 1

ER -

Quantification of cytochrome P450 reductase gene expression in human tissues

Abstract

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