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Abstract
T cell expansion and differentiation are critically dependent on the transcription factor c-Myc (Myc). Herein we use quantitative mass-spectrometry to reveal how Myc controls antigen receptor driven cell growth and proteome restructuring in murine T cells. Analysis of copy numbers per cell of >7000 proteins provides new understanding of the selective role of Myc in controlling the protein machinery that govern T cell fate. The data identify both Myc dependent and independent metabolic processes in immune activated T cells. We uncover that a primary function of Myc is to control expression of multiple amino acid transporters and that loss of a single Myc-controlled amino acid transporter effectively phenocopies the impact of Myc deletion. This study provides a comprehensive map of how Myc selectively shapes T cell phenotypes, revealing that Myc induction of amino acid transport is pivotal for subsequent bioenergetic and biosynthetic programs and licences T cell receptor driven proteome reprogramming.
Original language | English |
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Article number | e53725 |
Number of pages | 23 |
Journal | eLife |
Volume | 9 |
Early online date | 5 Feb 2020 |
DOIs | |
Publication status | Published - 4 Mar 2020 |
Keywords
- Myc
- T cell activation
- T lymphocyte
- amino acid transport
- immunology
- inflammation
- mouse
- proteomics
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Fluorescence Activated Cell Sorting for Cell Biology and Immunology (Equipment Grant)
1/09/16 → 1/12/19
Project: Research
Profiles
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Cantrell, Doreen
- Cell Signalling and Immunology - Wellcome Trust Principal Research Fellow & Immunology
Person: Academic