Projects per year
Quantitative mass spectrometry reveals how CD4 + and CD8 + T cells restructure proteomes in response to antigen and mammalian target of rapamycin complex 1 (mTORC1). Analysis of copy numbers per cell of >9,000 proteins provides new understanding of T cell phenotypes, exposing the metabolic and protein synthesis machinery and environmental sensors that shape T cell fate. We reveal that lymphocyte environment sensing is controlled by immune activation, and that CD4 + and CD8 + T cells differ in their intrinsic nutrient transport and biosynthetic capacity. Our data also reveal shared and divergent outcomes of mTORC1 inhibition in naïve versus effector T cells: mTORC1 inhibition impaired cell cycle progression in activated naïve cells, but not effector cells, whereas metabolism was consistently impacted in both populations. This study provides a comprehensive map of naïve and effector T cell proteomes, and a resource for exploring and understanding T cell phenotypes and cell context effects of mTORC1.
|Number of pages||13|
|Early online date||7 Oct 2019|
|Publication status||Published - Nov 2019|
ASJC Scopus subject areas
- Immunology and Allergy
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Fluorescence Activated Cell Sorting for Cell Biology and Immunology (Equipment Grant)
1/09/16 → 1/12/19
Multidimensional Proteomic Analysis of Metabolic Stress & Cellular Phenotypes (Strategic Grant)
1/01/15 → 31/12/19
- Cell Signalling and Immunology - Wellcome Trust Principal Research Fellow of Immunology