Quantitative analysis of the tomato nuclear proteome during Phytophthora capsici infection unveils regulators of immunity

Andrew J. M. Howden, Remco Stam, Victor Martinez Heredia, Graham B. Motion, Kelly Hodge, Sara ten Have, Tiago M. Marques Monteiro Amaro, Edgar Huitema (Lead / Corresponding author)

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Abstract

Plant–pathogen interactions are complex associations driven by the interplay of host and microbe-encoded factors. With secreted pathogen proteins (effectors) and immune signalling components found in the plant nucleus, this compartment is a battleground where susceptibility is specified. We hypothesized that, by defining changes in the nuclear proteome during infection, we can pinpoint vital components required for immunity or susceptibility. We tested this hypothesis by documenting dynamic changes in the tomato (Solanum lycopersicum) nuclear proteome during infection by the oomycete pathogen Phytophthora capsici. We enriched nuclei from infected and noninfected tissues and quantitatively assessed changes in the nuclear proteome. We then tested the role of candidate regulators in immunity through functional assays. We demonstrated that the host nuclear proteome dynamically changes during P. capsici infection. We observed that known nuclear immunity factors were differentially expressed and, based on this observation, selected a set of candidate regulators that we successfully implicated in immunity to P. capsici. Our work exemplifies a powerful strategy to gain rapid insight into important nuclear processes that underpin complex crop traits such as resistance. We have identified a large set of candidate nuclear factors that may underpin immunity to pathogens in crops.
Original languageEnglish
Pages (from-to)309-322
Number of pages14
JournalNew Phytologist
Volume215
Issue number1
Early online date10 Apr 2017
DOIs
Publication statusPublished - Jul 2017

Fingerprint

Phytophthora
Phytophthora capsici
Proteome
Lycopersicon esculentum
proteome
quantitative analysis
Immunity
immunity
tomatoes
Infection
infection
pathogens
Oomycetes
Solanum lycopersicum
crops
microorganisms
assays
Proteins
proteins

Keywords

  • immunity
  • nucleus
  • Phytophthora
  • plant-microbe interactions
  • quantitative proteomics
  • tomato

Cite this

Howden, Andrew J. M. ; Stam, Remco ; Martinez Heredia, Victor ; Motion, Graham B. ; Hodge, Kelly ; ten Have, Sara ; Marques Monteiro Amaro, Tiago M. ; Huitema, Edgar. / Quantitative analysis of the tomato nuclear proteome during Phytophthora capsici infection unveils regulators of immunity. In: New Phytologist. 2017 ; Vol. 215, No. 1. pp. 309-322.
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abstract = "Plant–pathogen interactions are complex associations driven by the interplay of host and microbe-encoded factors. With secreted pathogen proteins (effectors) and immune signalling components found in the plant nucleus, this compartment is a battleground where susceptibility is specified. We hypothesized that, by defining changes in the nuclear proteome during infection, we can pinpoint vital components required for immunity or susceptibility. We tested this hypothesis by documenting dynamic changes in the tomato (Solanum lycopersicum) nuclear proteome during infection by the oomycete pathogen Phytophthora capsici. We enriched nuclei from infected and noninfected tissues and quantitatively assessed changes in the nuclear proteome. We then tested the role of candidate regulators in immunity through functional assays. We demonstrated that the host nuclear proteome dynamically changes during P. capsici infection. We observed that known nuclear immunity factors were differentially expressed and, based on this observation, selected a set of candidate regulators that we successfully implicated in immunity to P. capsici. Our work exemplifies a powerful strategy to gain rapid insight into important nuclear processes that underpin complex crop traits such as resistance. We have identified a large set of candidate nuclear factors that may underpin immunity to pathogens in crops.",
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note = "This work was funded by the Biotechnology and Biological Sciences Research Council (BB/I00386X/1) and the European Research Council (310901_RETRaIN) and a Wellcome Trust strategic award (097945/2/11/2). Phytophthora capsici cultures were held at The James Hutton Institute under license PH\6\2015.",
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Quantitative analysis of the tomato nuclear proteome during Phytophthora capsici infection unveils regulators of immunity. / Howden, Andrew J. M.; Stam, Remco; Martinez Heredia, Victor; Motion, Graham B.; Hodge, Kelly; ten Have, Sara; Marques Monteiro Amaro, Tiago M.; Huitema, Edgar (Lead / Corresponding author).

In: New Phytologist, Vol. 215, No. 1, 07.2017, p. 309-322.

Research output: Contribution to journalArticle

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