@inbook{31f3c28799804526b0412830201b2e83,
title = "Quantitative Determination of Antibacterial Activity During Bacterial Coculture",
abstract = "Antibacterial activity assays are an important tool in the assessment of the ability of one bacterium to kill or inhibit the growth of another, for example, during the study of the Type VI secretion system (T6SS) and the antibacterial toxins it secretes. The method we describe here can detect the ability of a bacterial strain to kill or inhibit other bacterial cells in a contact-dependent manner when cocultured on an agar surface. It is particularly useful since it enumerates the recovery of viable target cells and thus enables quantification of the antibacterial activity. We provide a detailed description of how to measure the T6SS-dependent antibacterial activity of a bacterium such as Serratia marcescens against a competitor prokaryotic organism, Escherichia coli, and describe possible variations in the method to allow adaptation to other attacker and target organisms.",
keywords = "Coculture Techniques, Bacteria, Agar, Anti-Bacterial Agents/pharmacology, Biological Assay, Escherichia coli, Bacterial competitive fitness, Toxin/Immunity pair, Protein secretion system, Antibacterial activity, Coculture assay, Gram-negative bacteria, Type VI Secretion System",
author = "{Alcoforado Diniz}, Juliana and Christopher Earl and Hernandez, {Ruth E.} and Birte Hollmann and Coulthurst, {Sarah J.}",
note = "Copyright: {\textcopyright} 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2024",
doi = "10.1007/978-1-0716-3445-5_37",
language = "English",
isbn = "978071634448",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "593--600",
editor = "Laure Journet and Eric Casales",
booktitle = "Bacterial Secretion Systems",
address = "United States",
edition = "2",
}