Quantitative evaluation of the expression and activity of five major sulfotransferases (SULTs) in human tissues: the SULT "pie"

Zoe Riches, Emma L. Stanley, Jackie C. Bloomer, Michael W. H. Coughtrie

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    Abstract

    Expression levels of the major human sulfotransferases (SULTs) involved in xenobiotic detoxification in a range of human tissues (i.e., SULT "pies") are not available in a form allowing comparison between tissues and individuals. Here we have determined, by quantitative immunoblotting, expression levels for the five principal human SULTs-SULT1A1, SULT1A3/4, SULT1B1, SULT1E1, and SULT2A1-and determined the kinetic properties toward probe substrates, where available, for these enzymes in cytosol samples from a bank of adult human liver, small intestine, kidney, and lung. We produced new isoform-selective antibodies against SULT1B1 and SULT2A1, which were used alongside antibodies against SULT1A3 and SULT1A1 previously produced in our laboratory or available commercially (SULT1E1). Expression levels were derived using purified recombinant enzymes to construct standard curves for each individual isoform and immunoblot. Substantial intertissue and interindividual differences in expression were observed. SULT1A1 was the major enzyme (>50% of total, range 420-4900 ng/mg cytosol protein) in the liver, followed by SULT2A1, SULT1B1, and SULT1E1. SULT1A3 was completely absent from this tissue. In contrast, the small intestine contained the largest overall amount of SULT of any of the tissues, with SULT1B1 the major enzyme (36%), closely followed by SULT1A3 (31%), and SULT1A1, SULT1E1, and SULT2A1 more minor forms (19, 8, and 6% of total, respectively). The kidney and lung contained low levels of SULT. We provide a unique data set that will add value to the study of the role and contribution of sulfation to drug and xenobiotic metabolism in humans.

    Original languageEnglish
    Pages (from-to)2255-2261
    Number of pages7
    JournalDrug Metabolism and Disposition
    Volume37
    Issue number11
    Early online date13 Aug 2009
    DOIs
    Publication statusPublished - Nov 2009

    Keywords

    • Human cytosolic sulfotransferases
    • Human estrogen sulfotransferase
    • Human liver
    • Thyroid hormone
    • Hydroxysteroid sulfotransferase
    • Dehydroepiandrosterone sulfotransferase
    • Phenol sulfotransferases
    • Lithocholic acid
    • Rat brain
    • Identification

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