Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells

Gavuthami Murugesan; Lindsay Davidson; Linda Jannetti; Paul R. Crocker; Bernd Weigle

doi:10.3390/biomedicines10020239

Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells

Gavuthami Murugesan, Lindsay Davidson, Linda Jannetti, Paul R. Crocker, Bernd Weigle (Lead / Corresponding author)

Cell Signalling and Immunology

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

146 Downloads (Pure)

Abstract

Macrophages (M_Φ) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated M_Φ can exist in two extreme phenotypes: proinflammatory (M1) M_Φ and anti-inflammatory (M2) M_Φ. These phenotypes can be recapitulated in vitro by using ligands of toll-like receptors (TLRs) and cytokines such as IFNγ and IL-4. In recent years, human induced pluripotent stem cells (iPSC)-derived M_Φ have gained major attention, as they are functionally similar to human monocyte-derived M_Φ and are receptive to genome editing. In this study, we polarised iPSC-derived M_Φ to M1 or M2 and analysed their proteome and secretome profiles using quantitative proteomics. These comprehensive proteomic data sets provide new insights into functions of polarised M_Φ.

Original language	English
Article number	239
Pages (from-to)	1-19
Number of pages	19
Journal	Biomedicines
Volume	10
Issue number	2
DOIs	https://doi.org/10.3390/biomedicines10020239
Publication status	Published - 23 Jan 2022

Keywords

IPSC
Macrophages
Polarisation
Proteomics
Secretome

ASJC Scopus subject areas

Medicine (miscellaneous)
General Biochemistry,Genetics and Molecular Biology

Access to Document

10.3390/biomedicines10020239Licence: CC BY

Final published versionFinal published version, 4.98 MBLicence: CC BY

Cite this

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title = "Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells",

abstract = "Macrophages (MΦ) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated MΦ can exist in two extreme phenotypes: proinflammatory (M1) MΦ and anti-inflammatory (M2) MΦ. These phenotypes can be recapitulated in vitro by using ligands of toll-like receptors (TLRs) and cytokines such as IFNγ and IL-4. In recent years, human induced pluripotent stem cells (iPSC)-derived MΦ have gained major attention, as they are functionally similar to human monocyte-derived MΦ and are receptive to genome editing. In this study, we polarised iPSC-derived MΦ to M1 or M2 and analysed their proteome and secretome profiles using quantitative proteomics. These comprehensive proteomic data sets provide new insights into functions of polarised MΦ.",

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author = "Gavuthami Murugesan and Lindsay Davidson and Linda Jannetti and Crocker, {Paul R.} and Bernd Weigle",

note = "Funding Information: This research was funded by a grant from Boehringer Ingelheim (G.M.). We would like to thank Jeffrey Pollard and his group (University of Edinburgh) for their help with the iPSC differentiation protocol. We would like to thank Ignacio Moraga?s group for their help with obtaining buffy coats and isolating PBMCs. We would like to thank Abdelmadjid Atrih and others in the University of Dundee Proteomics facility for their assistance. We would like to thank the staff in the Flow Cytometry and Cell Sorting Facility at the University of Dundee for assistance.. Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

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AU - Weigle, Bernd

N1 - Funding Information: This research was funded by a grant from Boehringer Ingelheim (G.M.). We would like to thank Jeffrey Pollard and his group (University of Edinburgh) for their help with the iPSC differentiation protocol. We would like to thank Ignacio Moraga?s group for their help with obtaining buffy coats and isolating PBMCs. We would like to thank Abdelmadjid Atrih and others in the University of Dundee Proteomics facility for their assistance. We would like to thank the staff in the Flow Cytometry and Cell Sorting Facility at the University of Dundee for assistance.. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

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N2 - Macrophages (MΦ) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated MΦ can exist in two extreme phenotypes: proinflammatory (M1) MΦ and anti-inflammatory (M2) MΦ. These phenotypes can be recapitulated in vitro by using ligands of toll-like receptors (TLRs) and cytokines such as IFNγ and IL-4. In recent years, human induced pluripotent stem cells (iPSC)-derived MΦ have gained major attention, as they are functionally similar to human monocyte-derived MΦ and are receptive to genome editing. In this study, we polarised iPSC-derived MΦ to M1 or M2 and analysed their proteome and secretome profiles using quantitative proteomics. These comprehensive proteomic data sets provide new insights into functions of polarised MΦ.

AB - Macrophages (MΦ) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated MΦ can exist in two extreme phenotypes: proinflammatory (M1) MΦ and anti-inflammatory (M2) MΦ. These phenotypes can be recapitulated in vitro by using ligands of toll-like receptors (TLRs) and cytokines such as IFNγ and IL-4. In recent years, human induced pluripotent stem cells (iPSC)-derived MΦ have gained major attention, as they are functionally similar to human monocyte-derived MΦ and are receptive to genome editing. In this study, we polarised iPSC-derived MΦ to M1 or M2 and analysed their proteome and secretome profiles using quantitative proteomics. These comprehensive proteomic data sets provide new insights into functions of polarised MΦ.

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Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells

Abstract

Keywords

ASJC Scopus subject areas

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Flow Cytometry and Cell Sorting

Cite this

Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Equipment

Fingerprints Proteomics Facility

Flow Cytometry and Cell Sorting

Cite this