Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells

Gavuthami Murugesan, Lindsay Davidson, Linda Jannetti, Paul R. Crocker, Bernd Weigle (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
84 Downloads (Pure)

Abstract

Macrophages (MΦ) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated MΦ can exist in two extreme phenotypes: proinflammatory (M1) MΦ and anti-inflammatory (M2) MΦ. These phenotypes can be recapitulated in vitro by using ligands of toll-like receptors (TLRs) and cytokines such as IFNγ and IL-4. In recent years, human induced pluripotent stem cells (iPSC)-derived MΦ have gained major attention, as they are functionally similar to human monocyte-derived MΦ and are receptive to genome editing. In this study, we polarised iPSC-derived MΦ to M1 or M2 and analysed their proteome and secretome profiles using quantitative proteomics. These comprehensive proteomic data sets provide new insights into functions of polarised MΦ.

Original languageEnglish
Article number239
Pages (from-to)1-19
Number of pages19
JournalBiomedicines
Volume10
Issue number2
DOIs
Publication statusPublished - 23 Jan 2022

Keywords

  • IPSC
  • Macrophages
  • Polarisation
  • Proteomics
  • Secretome

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • General Biochemistry,Genetics and Molecular Biology

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