Abstract
Macrophages (MΦ) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated MΦ can exist in two extreme phenotypes: proinflammatory (M1) MΦ and anti-inflammatory (M2) MΦ. These phenotypes can be recapitulated in vitro by using ligands of toll-like receptors (TLRs) and cytokines such as IFNγ and IL-4. In recent years, human induced pluripotent stem cells (iPSC)-derived MΦ have gained major attention, as they are functionally similar to human monocyte-derived MΦ and are receptive to genome editing. In this study, we polarised iPSC-derived MΦ to M1 or M2 and analysed their proteome and secretome profiles using quantitative proteomics. These comprehensive proteomic data sets provide new insights into functions of polarised MΦ.
Original language | English |
---|---|
Article number | 239 |
Pages (from-to) | 1-19 |
Number of pages | 19 |
Journal | Biomedicines |
Volume | 10 |
Issue number | 2 |
DOIs | |
Publication status | Published - 23 Jan 2022 |
Keywords
- IPSC
- Macrophages
- Polarisation
- Proteomics
- Secretome
ASJC Scopus subject areas
- Medicine (miscellaneous)
- General Biochemistry,Genetics and Molecular Biology
Fingerprint
Dive into the research topics of 'Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells'. Together they form a unique fingerprint.Equipment
-
Fingerprints Proteomics Facility
Centre for Advanced Scientific TechnologiesFacility/equipment: Facility
-
Flow Cytometry and Cell Sorting
Centre for Advanced Scientific TechnologiesFacility/equipment: Facility