Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells

TY - JOUR

T1 - Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells

AU - Murugesan, Gavuthami

AU - Davidson, Lindsay

AU - Jannetti, Linda

AU - Crocker, Paul R.

AU - Weigle, Bernd

N1 - Funding Information: This research was funded by a grant from Boehringer Ingelheim (G.M.). We would like to thank Jeffrey Pollard and his group (University of Edinburgh) for their help with the iPSC differentiation protocol. We would like to thank Ignacio Moraga?s group for their help with obtaining buffy coats and isolating PBMCs. We would like to thank Abdelmadjid Atrih and others in the University of Dundee Proteomics facility for their assistance. We would like to thank the staff in the Flow Cytometry and Cell Sorting Facility at the University of Dundee for assistance.. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2022/1/23

Y1 - 2022/1/23

N2 - Macrophages (MΦ) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated MΦ can exist in two extreme phenotypes: proinflammatory (M1) MΦ and anti-inflammatory (M2) MΦ. These phenotypes can be recapitulated in vitro by using ligands of toll-like receptors (TLRs) and cytokines such as IFNγ and IL-4. In recent years, human induced pluripotent stem cells (iPSC)-derived MΦ have gained major attention, as they are functionally similar to human monocyte-derived MΦ and are receptive to genome editing. In this study, we polarised iPSC-derived MΦ to M1 or M2 and analysed their proteome and secretome profiles using quantitative proteomics. These comprehensive proteomic data sets provide new insights into functions of polarised MΦ.

AB - Macrophages (MΦ) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated MΦ can exist in two extreme phenotypes: proinflammatory (M1) MΦ and anti-inflammatory (M2) MΦ. These phenotypes can be recapitulated in vitro by using ligands of toll-like receptors (TLRs) and cytokines such as IFNγ and IL-4. In recent years, human induced pluripotent stem cells (iPSC)-derived MΦ have gained major attention, as they are functionally similar to human monocyte-derived MΦ and are receptive to genome editing. In this study, we polarised iPSC-derived MΦ to M1 or M2 and analysed their proteome and secretome profiles using quantitative proteomics. These comprehensive proteomic data sets provide new insights into functions of polarised MΦ.

KW - IPSC

KW - Macrophages

KW - Polarisation

KW - Proteomics

KW - Secretome

UR - https://www.scopus.com/pages/publications/85123602872

U2 - 10.3390/biomedicines10020239

DO - 10.3390/biomedicines10020239

M3 - Article

C2 - 35203449

AN - SCOPUS:85123602872

SN - 2227-9059

VL - 10

SP - 1

EP - 19

JO - Biomedicines

JF - Biomedicines

IS - 2

M1 - 239

ER -