Quasi-noise-free stimulated emission depletion microscopy imaging of thick samples using adaptive optics and blockmatching 3D filtering

Piotr Zdankowski (Lead / Corresponding author), Maciej Trusiak, David McGloin, Jason R. Swedlow

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a novel-method of increasing signal-to-noise-ratio and effective-resolution of STED microscope by combining aberration-correction and image-processing. We imaged 15μm thick mitotic cell and observed in-plane resolution of 118nm without filtering and 70nm with filtering.

Original languageEnglish
Title of host publicationProceedings Imaging Systems and Applications (ISA 2019)
PublisherOptical Society of America
ISBN (Print)9781943580637
DOIs
Publication statusPublished - 2019
EventImaging Systems and Applications, ISA 2019 - Munich, Germany
Duration: 24 Jun 201927 Jun 2019

Publication series

NameOptics InfoBase Conference Papers
VolumePart F157-ISA 2019

Conference

ConferenceImaging Systems and Applications, ISA 2019
CountryGermany
CityMunich
Period24/06/1927/06/19

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  • Cite this

    Zdankowski, P., Trusiak, M., McGloin, D., & Swedlow, J. R. (2019). Quasi-noise-free stimulated emission depletion microscopy imaging of thick samples using adaptive optics and blockmatching 3D filtering. In Proceedings Imaging Systems and Applications (ISA 2019) [ITh1C.4] (Optics InfoBase Conference Papers; Vol. Part F157-ISA 2019). Optical Society of America. https://doi.org/10.1364/ISA.2019.ITh1C.4