RAB1A promotes Vaccinia virus replication by facilitating the production of intracellular enveloped virions

Tali Pechenick Jowers, Rebecca J. Featherstone, Danielle K. Reynolds, Helen K. Brown, John James, Alan Prescott, Ismar R. Haga, Philippa M. Beard (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    Vaccinia virus (VACV) is a large double-stranded DNA virus with a complex cytoplasmic replication cycle that exploits numerous cellular proteins. This work characterises the role of a proviral cellular protein, the small GTPase RAB1A, in VACV replication. Using siRNA, we identified RAB1A as required for the production of extracellular enveloped virions (EEVs), but not intracellular mature virions (IMVs). Immunofluorescence and electron microscopy further refined the role of RAB1A as facilitating the wrapping of IMVs to become intracellular enveloped virions (IEVs). This is consistent with the known function of RAB1A in maintenance of ER to Golgi transport. VACV can therefore be added to the growing list of viruses which require RAB1A for optimal replication, highlighting this protein as a broadly proviral host factor.

    Original languageEnglish
    Pages (from-to)66-73
    Number of pages8
    JournalVirology
    Volume475
    DOIs
    Publication statusPublished - 15 Jan 2015

    Fingerprint

    Vaccinia virus
    Virus Replication
    Virion
    Proteins
    DNA Viruses
    Monomeric GTP-Binding Proteins
    Fluorescence Microscopy
    Small Interfering RNA
    Electron Microscopy
    Maintenance
    Viruses
    DNA

    Cite this

    Pechenick Jowers, T., Featherstone, R. J., Reynolds, D. K., Brown, H. K., James, J., Prescott, A., ... Beard, P. M. (2015). RAB1A promotes Vaccinia virus replication by facilitating the production of intracellular enveloped virions. Virology, 475, 66-73. https://doi.org/10.1016/j.virol.2014.11.007
    Pechenick Jowers, Tali ; Featherstone, Rebecca J. ; Reynolds, Danielle K. ; Brown, Helen K. ; James, John ; Prescott, Alan ; Haga, Ismar R. ; Beard, Philippa M. / RAB1A promotes Vaccinia virus replication by facilitating the production of intracellular enveloped virions. In: Virology. 2015 ; Vol. 475. pp. 66-73.
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    abstract = "Vaccinia virus (VACV) is a large double-stranded DNA virus with a complex cytoplasmic replication cycle that exploits numerous cellular proteins. This work characterises the role of a proviral cellular protein, the small GTPase RAB1A, in VACV replication. Using siRNA, we identified RAB1A as required for the production of extracellular enveloped virions (EEVs), but not intracellular mature virions (IMVs). Immunofluorescence and electron microscopy further refined the role of RAB1A as facilitating the wrapping of IMVs to become intracellular enveloped virions (IEVs). This is consistent with the known function of RAB1A in maintenance of ER to Golgi transport. VACV can therefore be added to the growing list of viruses which require RAB1A for optimal replication, highlighting this protein as a broadly proviral host factor.",
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    Pechenick Jowers, T, Featherstone, RJ, Reynolds, DK, Brown, HK, James, J, Prescott, A, Haga, IR & Beard, PM 2015, 'RAB1A promotes Vaccinia virus replication by facilitating the production of intracellular enveloped virions', Virology, vol. 475, pp. 66-73. https://doi.org/10.1016/j.virol.2014.11.007

    RAB1A promotes Vaccinia virus replication by facilitating the production of intracellular enveloped virions. / Pechenick Jowers, Tali; Featherstone, Rebecca J.; Reynolds, Danielle K.; Brown, Helen K.; James, John; Prescott, Alan; Haga, Ismar R.; Beard, Philippa M. (Lead / Corresponding author).

    In: Virology, Vol. 475, 15.01.2015, p. 66-73.

    Research output: Contribution to journalArticle

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    AU - Featherstone, Rebecca J.

    AU - Reynolds, Danielle K.

    AU - Brown, Helen K.

    AU - James, John

    AU - Prescott, Alan

    AU - Haga, Ismar R.

    AU - Beard, Philippa M.

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