RAB1A promotes Vaccinia virus replication by facilitating the production of intracellular enveloped virions

Tali Pechenick Jowers; Rebecca J. Featherstone; Danielle K. Reynolds; Helen K. Brown; John James; Alan Prescott; Ismar R. Haga; Philippa M. Beard

doi:10.1016/j.virol.2014.11.007

RAB1A promotes Vaccinia virus replication by facilitating the production of intracellular enveloped virions

Tali Pechenick Jowers
, Rebecca J. Featherstone
, Danielle K. Reynolds
, Helen K. Brown
, John James
, Alan Prescott
, Ismar R. Haga
, Philippa M. Beard (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Vaccinia virus (VACV) is a large double-stranded DNA virus with a complex cytoplasmic replication cycle that exploits numerous cellular proteins. This work characterises the role of a proviral cellular protein, the small GTPase RAB1A, in VACV replication. Using siRNA, we identified RAB1A as required for the production of extracellular enveloped virions (EEVs), but not intracellular mature virions (IMVs). Immunofluorescence and electron microscopy further refined the role of RAB1A as facilitating the wrapping of IMVs to become intracellular enveloped virions (IEVs). This is consistent with the known function of RAB1A in maintenance of ER to Golgi transport. VACV can therefore be added to the growing list of viruses which require RAB1A for optimal replication, highlighting this protein as a broadly proviral host factor.

Original language	English
Pages (from-to)	66-73
Number of pages	8
Journal	Virology
Volume	475
DOIs	https://doi.org/10.1016/j.virol.2014.11.007
Publication status	Published - 15 Jan 2015

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1016/j.virol.2014.11.007

Strategic Award: Wellcome Trust Technology Platform
Blow, J. (Investigator), Lamond, A. (Investigator) & Owen-Hughes, T. (Investigator)
1/01/13 → 30/09/18
Project: Research

Cite this

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title = "RAB1A promotes Vaccinia virus replication by facilitating the production of intracellular enveloped virions",

abstract = "Vaccinia virus (VACV) is a large double-stranded DNA virus with a complex cytoplasmic replication cycle that exploits numerous cellular proteins. This work characterises the role of a proviral cellular protein, the small GTPase RAB1A, in VACV replication. Using siRNA, we identified RAB1A as required for the production of extracellular enveloped virions (EEVs), but not intracellular mature virions (IMVs). Immunofluorescence and electron microscopy further refined the role of RAB1A as facilitating the wrapping of IMVs to become intracellular enveloped virions (IEVs). This is consistent with the known function of RAB1A in maintenance of ER to Golgi transport. VACV can therefore be added to the growing list of viruses which require RAB1A for optimal replication, highlighting this protein as a broadly proviral host factor.",

author = "\{Pechenick Jowers\}, Tali and Featherstone, \{Rebecca J.\} and Reynolds, \{Danielle K.\} and Brown, \{Helen K.\} and John James and Alan Prescott and Haga, \{Ismar R.\} and Beard, \{Philippa M.\}",

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doi = "10.1016/j.virol.2014.11.007",

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T1 - RAB1A promotes Vaccinia virus replication by facilitating the production of intracellular enveloped virions

AU - Pechenick Jowers, Tali

AU - Featherstone, Rebecca J.

AU - Reynolds, Danielle K.

AU - Brown, Helen K.

AU - James, John

AU - Prescott, Alan

AU - Haga, Ismar R.

AU - Beard, Philippa M.

PY - 2015/1/15

Y1 - 2015/1/15

N2 - Vaccinia virus (VACV) is a large double-stranded DNA virus with a complex cytoplasmic replication cycle that exploits numerous cellular proteins. This work characterises the role of a proviral cellular protein, the small GTPase RAB1A, in VACV replication. Using siRNA, we identified RAB1A as required for the production of extracellular enveloped virions (EEVs), but not intracellular mature virions (IMVs). Immunofluorescence and electron microscopy further refined the role of RAB1A as facilitating the wrapping of IMVs to become intracellular enveloped virions (IEVs). This is consistent with the known function of RAB1A in maintenance of ER to Golgi transport. VACV can therefore be added to the growing list of viruses which require RAB1A for optimal replication, highlighting this protein as a broadly proviral host factor.

AB - Vaccinia virus (VACV) is a large double-stranded DNA virus with a complex cytoplasmic replication cycle that exploits numerous cellular proteins. This work characterises the role of a proviral cellular protein, the small GTPase RAB1A, in VACV replication. Using siRNA, we identified RAB1A as required for the production of extracellular enveloped virions (EEVs), but not intracellular mature virions (IMVs). Immunofluorescence and electron microscopy further refined the role of RAB1A as facilitating the wrapping of IMVs to become intracellular enveloped virions (IEVs). This is consistent with the known function of RAB1A in maintenance of ER to Golgi transport. VACV can therefore be added to the growing list of viruses which require RAB1A for optimal replication, highlighting this protein as a broadly proviral host factor.

U2 - 10.1016/j.virol.2014.11.007

DO - 10.1016/j.virol.2014.11.007

M3 - Article

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SN - 0042-6822

VL - 475

SP - 66

EP - 73

JO - Virology

JF - Virology

ER -

RAB1A promotes Vaccinia virus replication by facilitating the production of intracellular enveloped virions

Abstract

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Projects

Strategic Award: Wellcome Trust Technology Platform

Cite this