Rab28 function in trypanosomes: interactions with retromer and ESCRT pathways

Jennifer H. Lumb, Ka Fai Leung, Kelly N. Dubois, Mark C. Field

    Research output: Contribution to journalArticlepeer-review

    28 Citations (Scopus)

    Abstract

    Early endosomal cargo is typically targeted to either a degradative or recycling pathway. Despite established functions for the retromer and ESCRT complexes at late endosomes/multivesicular bodies, the mechanisms integrating and coordinating these functions remain largely unknown. Rab family GTPases are key membrane trafficking organizers and could contribute. Here, in the unicellular organism Trypanosoma brucei, we demonstrate that Rab28 locates to the endosomal pathway and partially colocalizes with Vps23, an ESCRT I component. Rab28 is required for turnover of endocytosed proteins and for lysosomal delivery of protein cargo. Using RNA interference we find that in Rab28-depleted cells, protein levels of ESCRT I (Vps23/28) and retromer (Vps26) are also decreased, suggesting that Rab28 is an important regulator of these factors. We suggest that Rab28 coordinates the activity of retromer-dependent trafficking and ESCRT-mediated degradative pathways.
    Original languageEnglish
    Pages (from-to)3771-3783
    Number of pages13
    JournalJournal of Cell Science
    Volume124
    Issue numberP22
    DOIs
    Publication statusPublished - 15 Nov 2011

    Keywords

    • Protozoan Proteins
    • rab GTP-Binding Proteins
    • Vesicular Transport Proteins
    • Trypanosoma brucei brucei
    • Protein Binding
    • Endosomes
    • Endosomal Sorting Complexes Required for Transport

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