Rab6 and the secretory pathway affect oocyte polarity in Drosophila

Jens Januschke, Emmanuelle Nicolas, Julien Compagnon, Etienne Formstecher, Bruno Goud, Antoine Guichet

    Research output: Contribution to journalArticle

    44 Citations (Scopus)

    Abstract

    The Drosophila oocyte is a highly polarized cell. Secretion occurs towards restricted neighboring cells and asymmetric transport controls the localization of several mRNAs to distinct cortical compartments. Here, we describe a role for the Drosophila ortholog of the Rab6 GTPase, Drab6, in establishing cell polarity during oogenesis. We found that Drab6 localizes to Golgi and Golgi-derived membranes and interacts with BicD. We also provide evidence that Drab6 and BicD function together to ensure the correct delivery of secretory pathway components, such as the TGFalpha homolog Gurken, to the plasma membrane. Moreover, in the absence of Drab6, osk mRNA localization and the organization of microtubule plus-ends at the posterior of the oocyte were both severely affected. Our results point to a possible connection between Rab protein-mediated secretion, organization of the cytoskeleton and mRNA transport.
    Original languageEnglish
    Pages (from-to)3419-3425
    Number of pages7
    JournalDevelopment
    Volume134
    Issue number19
    DOIs
    Publication statusPublished - 2007

    Fingerprint

    Secretory Pathway
    Drosophila
    Oocytes
    Messenger RNA
    Secretory Component
    Oogenesis
    Cell Polarity
    Transforming Growth Factor alpha
    GTP Phosphohydrolases
    Cytoskeleton
    Microtubules
    Cell Membrane
    Membranes
    Proteins

    Cite this

    Januschke, J., Nicolas, E., Compagnon, J., Formstecher, E., Goud, B., & Guichet, A. (2007). Rab6 and the secretory pathway affect oocyte polarity in Drosophila. Development, 134(19), 3419-3425. https://doi.org/10.1242/dev.008078
    Januschke, Jens ; Nicolas, Emmanuelle ; Compagnon, Julien ; Formstecher, Etienne ; Goud, Bruno ; Guichet, Antoine. / Rab6 and the secretory pathway affect oocyte polarity in Drosophila. In: Development. 2007 ; Vol. 134, No. 19. pp. 3419-3425.
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    abstract = "The Drosophila oocyte is a highly polarized cell. Secretion occurs towards restricted neighboring cells and asymmetric transport controls the localization of several mRNAs to distinct cortical compartments. Here, we describe a role for the Drosophila ortholog of the Rab6 GTPase, Drab6, in establishing cell polarity during oogenesis. We found that Drab6 localizes to Golgi and Golgi-derived membranes and interacts with BicD. We also provide evidence that Drab6 and BicD function together to ensure the correct delivery of secretory pathway components, such as the TGFalpha homolog Gurken, to the plasma membrane. Moreover, in the absence of Drab6, osk mRNA localization and the organization of microtubule plus-ends at the posterior of the oocyte were both severely affected. Our results point to a possible connection between Rab protein-mediated secretion, organization of the cytoskeleton and mRNA transport.",
    author = "Jens Januschke and Emmanuelle Nicolas and Julien Compagnon and Etienne Formstecher and Bruno Goud and Antoine Guichet",
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    Januschke, J, Nicolas, E, Compagnon, J, Formstecher, E, Goud, B & Guichet, A 2007, 'Rab6 and the secretory pathway affect oocyte polarity in Drosophila', Development, vol. 134, no. 19, pp. 3419-3425. https://doi.org/10.1242/dev.008078

    Rab6 and the secretory pathway affect oocyte polarity in Drosophila. / Januschke, Jens; Nicolas, Emmanuelle; Compagnon, Julien; Formstecher, Etienne; Goud, Bruno; Guichet, Antoine.

    In: Development, Vol. 134, No. 19, 2007, p. 3419-3425.

    Research output: Contribution to journalArticle

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    AU - Guichet, Antoine

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    Januschke J, Nicolas E, Compagnon J, Formstecher E, Goud B, Guichet A. Rab6 and the secretory pathway affect oocyte polarity in Drosophila. Development. 2007;134(19):3419-3425. https://doi.org/10.1242/dev.008078