RAC1-Dependent ORAI1 Translocation to the Leading Edge Supports Lamellipodia Formation and Directional Persistence

Aida M. Lopez-Guerrero, Noelia Espinosa-Bermejo, Irene Sanchez-Lopez, Thomas Macartney, Carlos Pascual-Caro, Yolanda Orantos-Aguilera, Lola Rodriguez-Ruiz, Ana B. Perez-Oliva, Victoriano Mulero, Eulalia Pozo-Guisado (Lead / Corresponding author), Francisco Javier Martin-Romero (Lead / Corresponding author)

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Tumor invasion requires efficient cell migration, which is achieved by the generation of persistent and polarized lamellipodia. The generation of lamellipodia is supported by actin dynamics at the leading edge where a complex of proteins known as the WAVE regulatory complex (WRC) promotes the required assembly of actin filaments to push the front of the cell ahead. By using an U2OS osteosarcoma cell line with high metastatic potential, proven by a xenotransplant in zebrafish larvae, we have studied the role of the plasma membrane Ca2+ channel ORAI1 in this process. We have found that epidermal growth factor (EGF) triggered an enrichment of ORAI1 at the leading edge, where colocalized with cortactin (CTTN) and other members of the WRC, such as CYFIP1 and ARP2/3. ORAI1-CTTN co-precipitation was sensitive to the inhibition of the small GTPase RAC1, an upstream activator of the WRC. RAC1 potentiated ORAI1 translocation to the leading edge, increasing the availability of surface ORAI1 and increasing the plasma membrane ruffling. The role of ORAI1 at the leading edge was studied in genetically engineered U2OS cells lacking ORAI1 expression that helped us to prove the key role of this Ca2+ channel on lamellipodia formation, lamellipodial persistence, and cell directness, which are required for tumor cell invasiveness in vivo.

Original languageEnglish
Article number6580
Pages (from-to)1-18
Number of pages18
JournalScientific Reports
Publication statusPublished - 20 Apr 2020


  • Calcium signalling
  • Lamellipodia

ASJC Scopus subject areas

  • General


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