Raman spectroscopy for accurately characterizing biomolecular changes in androgen-independent prostate cancer cells

Stella Corsetti (Lead / Corresponding author), Thomas Rabl, David McGloin, Ghulam Nabi

Research output: Contribution to journalArticle

8 Citations (Scopus)
54 Downloads (Pure)

Abstract

Metastatic prostate cancer resistant to hormonal manipulation is considered the advanced stage of the disease and leads to most cancer related mortality. With new research focusing on modulating cancer growth, it is essential to understand the biochemical changes in cells that can then be exploited for drug discovery and for improving responsiveness to treatment. Raman spectroscopy has a high chemical specificity and can be used to detect and quantify molecular changes at the cellular level. Collection of large datasets generated from biological samples can be employed to form discriminatory algorithms for detection of subtle and early changes in cancer cells. The present study describes Raman finger-printing of normal and metastatic hormone-resistant prostate cancer cells including analyses with principal component analysis and linear discrimination. Amino acid-specific signals were identified, especially loss of arginine band. Androgen-resistant prostate cancer cells presented a higher content of phenylalanine, tyrosine, DNA and Amide III in comparison to PNT2 cells, which possessed greater amounts of L-arginine and had a B conformation of DNA. The analysis utilised in this study could reliably differentiate the two cell lines (sensitivity 95%; specificity 88%).
Original languageEnglish
Article numbere201700166
Pages (from-to)1-8
Number of pages8
JournalJournal of Biophotonics
Volume11
Issue number3
Early online date19 Sep 2017
DOIs
Publication statusPublished - 23 Nov 2017

Keywords

  • metastatic prostate cancer cells
  • castrate resistant prostate cancer (CRPC)
  • Raman spectroscopy
  • phenylalanine
  • L-arginine

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  • Student Theses

    Spontaneous Raman spectroscopy: Exploring applicability in drug discovery and the medical sciences

    Author: Rabl, T., 2018

    Supervisor: Read, K. (Supervisor) & McGloin, D. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy

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    Nabi, Ghulam

    Person: Academic

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