Ran alters nuclear pore complex conformation

M. W. Goldberg (Lead / Corresponding author), S. A. Rutherford, M. Hughes, L. A. Cotter, S. Bagley, Elena Kiseleva, Terence D. Allen, P. R. Clarke

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Transport across the nuclear membranes occurs through the nuclear pore complex (NPC), and is mediated by soluble transport factors including Ran, a small GTPase that is generally GDP-bound during import and GTP-bound for export. The dynamic nature of the NPC structure suggests a possible active role for it in driving translocation. Here we show that RanGTP but not RanGDP causes alterations of NPC structure when injected into the cytoplasm of Xenopus oocytes, including compaction of the NPC and extension of the cytoplasmic filaments. RanGTP caused accumulation of nucleoplasmin-gold along the length of extended cytoplasmic filaments, whereas RanGDP caused accumulation around the cytoplasmic rim of the NPC. This suggests a possible role for Ran in altering the conformation of the cytoplasmic filaments during transport. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)519-529
Number of pages11
JournalJournal of Molecular Biology
Volume300
Issue number3
DOIs
Publication statusPublished - 14 Jul 2000

    Fingerprint

Keywords

  • Nuclear pore complex
  • Ran
  • Transport

Cite this

Goldberg, M. W., Rutherford, S. A., Hughes, M., Cotter, L. A., Bagley, S., Kiseleva, E., Allen, T. D., & Clarke, P. R. (2000). Ran alters nuclear pore complex conformation. Journal of Molecular Biology, 300(3), 519-529. https://doi.org/10.1006/jmbi.2000.3891