Rapid protein kinase D translocation in response to G protein-coupled receptor activation: dependence on protein kinase C

Osvaldo Rey, Steven H. Young, Doreen Cantrell, Enrique Rozengurt (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    94 Citations (Scopus)

    Abstract

    Protein kinase D (PKD)/protein kinase C (PKC) μ is a serine/threonine protein kinase that can be activated by physiological stimuli like growth factors, antigen-receptor engagement and G protein-coupled receptor (GPCR) agonists via a phosphorylation-dependent mechanism that requires PKC activity. In order to investigate the dynamic mechanisms associated with GPCR signaling, the intracellular translocation of a green fluorescent protein-tagged PKD was analyzed by real-time visualization in fibroblasts and epithelial cells stimulated with bombesin, a GPCR agonist. We found that bombesin induced a rapidly reversible plasma membrane translocation of green fluorescent protein-tagged PKD, an event that can be divided into two distinct mechanistic steps. The first step, which is exclusively mediated by the cysteine-rich domain in the N terminus of PKD, involved its translocation from the cytosol to the plasma membrane. The second step, i.e. the rapid reverse translocation of PKD from the plasma membrane to the cytosol, required its catalytic domain and surprisingly PKC activity. These findings provide evidence for a novel mechanism by which PKC coordinates the translocation and activation of PKD in response to bombesin-induced GPCR activation.

    Original languageEnglish
    Pages (from-to)32616-32626
    Number of pages11
    JournalJournal of Biological Chemistry
    Volume276
    Issue number35
    DOIs
    Publication statusPublished - 31 Aug 2001

    Fingerprint Dive into the research topics of 'Rapid protein kinase D translocation in response to G protein-coupled receptor activation: dependence on protein kinase C'. Together they form a unique fingerprint.

  • Cite this