Rapid, Transcript-Specific Changes in Splicing in Response to Environmental Stress

Jeffrey A. Pleiss, Gregg B. Whitworth, Megan Bergkessel, Christine Guthrie (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

150 Citations (Scopus)

Abstract

While the core splicing machinery is highly conserved between budding yeast and mammals, the absence of alternative splicing in Saccharomyces cerevisiae raises the fundamental question of why introns have been retained in ∼5% of the 6000 genes. Because ribosomal protein-encoding genes (RPGs) are highly overrepresented in the set of intron-containing genes, we tested the hypothesis that splicing of these transcripts would be regulated under conditions in which translation is impaired. Using a microarray-based strategy, we find that, within minutes after the induction of amino acid starvation, the splicing of the majority of RPGs is specifically inhibited. In response to an unrelated stress, exposure to toxic levels of ethanol, splicing of a different group of transcripts is inhibited, while the splicing of a third set is actually improved. We propose that regulation of splicing, like transcription, can afford rapid and specific changes in gene expression in response to the environment.

Original languageEnglish
Pages (from-to)928-937
Number of pages10
JournalMolecular Cell
Volume27
Issue number6
Early online date20 Sep 2007
DOIs
Publication statusPublished - 21 Sep 2007

Keywords

  • RNA
  • Signaling

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