RASSF7 is a member of a new family of RAS association domain-containing proteins and is required for completing mitosis

Victoria Sherwood, Ria Manbodh, Carol Sheppard, Andrew D. Chalmers (Lead / Corresponding author)

    Research output: Contribution to journalArticlepeer-review

    48 Citations (Scopus)

    Abstract

    Mitosis is a fundamental feature of all cellular organisms. It must be tightly regulated to allow normal tissue growth and to prevent cancer formation. Here, we identify a new protein that is required for mitosis. We show that the Ras association (RA) domain-containing protein, RASSF7, is part of an evolutionarily conserved group of four proteins. These are RASSF7, RASSF8, and two new RASSF proteins P-CIP1/RASSF9 and RASSF10. We call this group the N-terminal RASSF family. We analyzed the function of Xenopus RASSF7. RASSF7 was found to be expressed in several embryonic tissues including the skin, eyes, and neural tube. Knocking down its function led to cells failing to form a mitotic spindle and arresting in mitosis. This caused nuclear breakdown, apoptosis, and a striking loss of tissue architecture in the neural tube. Consistent with a role in spindle formation, RASSF7 protein was found to localize to the centrosome. This localization occurred in a microtubule-dependent manner, demonstrating that there is a mutually dependant relationship between RASSF7 localization and spindle formation. Thus RASSF7, the first member of the N-terminal RASSF family to be functionally analyzed, is a centrosome-associated protein required to form a spindle and complete mitosis in the neural tube.

    Original languageEnglish
    Pages (from-to)1772-1782
    Number of pages11
    JournalMolecular Biology of the Cell
    Volume19
    Issue number4
    DOIs
    Publication statusPublished - Apr 2008

    Keywords

    • Animals
    • Apoptosis
    • Base sequence
    • Centrosome
    • DNA primers
    • Drosophila proteins
    • Evolution, Molecular
    • Gene expression regulation, Developmental
    • Gene silencing
    • Humans
    • Mitosis
    • Monomeric GTP-binding proteins
    • Neural tube defects
    • Spindle apparatus
    • Xenopus
    • Xenopus proteins

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