Rat liver glutathione S-transferases. A study of the structure of the basic YbYb-containing enzymes

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    Abstract

    A purification scheme was devised that resulted in the resolution of a number of basic glutathione S-transferases from rat liver, three of which contained two subunits of molecular mass 23500 Da (i.e. Yb monomers). These were identified as transferases D, C and A by their elution positions from CM-cellulose and their specific activities towards a variety of substrates. Hybridization, immunotitration and peptide 'mapping' experiments demonstrated that transferases D, C and A comprise Yb2Yb2, Yb1Yb2 and Yb1Yb1 subunits.

    Original languageEnglish
    Pages (from-to)625-33
    Number of pages9
    JournalBiochemical Journal
    Volume213
    Issue number3
    Publication statusPublished - 1 Sep 1983

    Keywords

    • Amino Acids/analysis
    • Animals
    • Electrophoresis, Paper
    • Glutathione Transferase/isolation & purification
    • Isoenzymes/isolation & purification
    • Liver/enzymology
    • Macromolecular Substances
    • Peptide Fragments/analysis
    • Rats
    • Substrate Specificity

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