Real-time RT-PCR threshold cycles value for Kir6.1 from the blood correlates with parameters of vascular function: a potential for the vascular function biomarker?

Faisel Khan, Wen Ling Choong, Qingyou Du, Aleksandar Jovanovic

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    Abstract We examined the presence of KATP channel subunits, Kir6.1 and SUR2B, mRNAs in the blood and vascular function in healthy volunteers (41 males, 34 females). Real-time reverse transcriptase (RT)-PCR threshold cycles (Ct) was used as an indicator of mRNA levels. Baseline skin perfusion and the post-occlusion reactive hyperemia response exhibited a significant positive correlation with Ct for Kir6.1. There was no correlation between Kir6.1 Ct and brachial artery flow-mediated dilatation. Gender had no influence on relationships between blood Kir6.1 Ct and vascular function. We conclude that blood Kir6.1 mRNA levels could be potentially used as a biomarker of the vascular function.
    Original languageEnglish
    Pages (from-to)221-229
    Number of pages9
    JournalBiomarkers
    Volume18
    Issue number3
    DOIs
    Publication statusPublished - May 2013

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