Real-time RT-PCR threshold cycles value for Kir6.1 from the blood correlates with parameters of vascular function: a potential for the vascular function biomarker?

Faisel Khan; Wen Ling Choong; Qingyou Du; Aleksandar Jovanovic

doi:10.3109/1354750X.2013.770553

Real-time RT-PCR threshold cycles value for Kir6.1 from the blood correlates with parameters of vascular function: a potential for the vascular function biomarker?

Faisel Khan, Wen Ling Choong, Qingyou Du, Aleksandar Jovanovic

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Abstract We examined the presence of KATP channel subunits, Kir6.1 and SUR2B, mRNAs in the blood and vascular function in healthy volunteers (41 males, 34 females). Real-time reverse transcriptase (RT)-PCR threshold cycles (Ct) was used as an indicator of mRNA levels. Baseline skin perfusion and the post-occlusion reactive hyperemia response exhibited a significant positive correlation with Ct for Kir6.1. There was no correlation between Kir6.1 Ct and brachial artery flow-mediated dilatation. Gender had no influence on relationships between blood Kir6.1 Ct and vascular function. We conclude that blood Kir6.1 mRNA levels could be potentially used as a biomarker of the vascular function.

Original language	English
Pages (from-to)	221-229
Number of pages	9
Journal	Biomarkers
Volume	18
Issue number	3
DOIs	https://doi.org/10.3109/1354750X.2013.770553
Publication status	Published - May 2013

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10.3109/1354750X.2013.770553

Regulation of Subsarcolemmal ATP by Subsarcolemmal KATP Channels: Is it Important for Cardioprotection
Jovanovic, A. (Investigator)
British Heart Foundation
18/01/13 → 17/01/16
Project: Research

Determining whether plasma levels of KATP channels are related to vascular function
Choong, W. L. (Author), Khan, F. (Supervisor) & Jovanovic, A. (Supervisor), 2013
Student thesis: Master's Thesis › Master of Science

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title = "Real-time RT-PCR threshold cycles value for Kir6.1 from the blood correlates with parameters of vascular function: a potential for the vascular function biomarker?",

abstract = "Abstract We examined the presence of KATP channel subunits, Kir6.1 and SUR2B, mRNAs in the blood and vascular function in healthy volunteers (41 males, 34 females). Real-time reverse transcriptase (RT)-PCR threshold cycles (Ct) was used as an indicator of mRNA levels. Baseline skin perfusion and the post-occlusion reactive hyperemia response exhibited a significant positive correlation with Ct for Kir6.1. There was no correlation between Kir6.1 Ct and brachial artery flow-mediated dilatation. Gender had no influence on relationships between blood Kir6.1 Ct and vascular function. We conclude that blood Kir6.1 mRNA levels could be potentially used as a biomarker of the vascular function.",

author = "Faisel Khan and Choong, {Wen Ling} and Qingyou Du and Aleksandar Jovanovic",

year = "2013",

month = may,

doi = "10.3109/1354750X.2013.770553",

language = "English",

volume = "18",

pages = "221--229",

journal = "Biomarkers",

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T1 - Real-time RT-PCR threshold cycles value for Kir6.1 from the blood correlates with parameters of vascular function

T2 - a potential for the vascular function biomarker?

AU - Khan, Faisel

AU - Choong, Wen Ling

AU - Du, Qingyou

AU - Jovanovic, Aleksandar

PY - 2013/5

Y1 - 2013/5

N2 - Abstract We examined the presence of KATP channel subunits, Kir6.1 and SUR2B, mRNAs in the blood and vascular function in healthy volunteers (41 males, 34 females). Real-time reverse transcriptase (RT)-PCR threshold cycles (Ct) was used as an indicator of mRNA levels. Baseline skin perfusion and the post-occlusion reactive hyperemia response exhibited a significant positive correlation with Ct for Kir6.1. There was no correlation between Kir6.1 Ct and brachial artery flow-mediated dilatation. Gender had no influence on relationships between blood Kir6.1 Ct and vascular function. We conclude that blood Kir6.1 mRNA levels could be potentially used as a biomarker of the vascular function.

AB - Abstract We examined the presence of KATP channel subunits, Kir6.1 and SUR2B, mRNAs in the blood and vascular function in healthy volunteers (41 males, 34 females). Real-time reverse transcriptase (RT)-PCR threshold cycles (Ct) was used as an indicator of mRNA levels. Baseline skin perfusion and the post-occlusion reactive hyperemia response exhibited a significant positive correlation with Ct for Kir6.1. There was no correlation between Kir6.1 Ct and brachial artery flow-mediated dilatation. Gender had no influence on relationships between blood Kir6.1 Ct and vascular function. We conclude that blood Kir6.1 mRNA levels could be potentially used as a biomarker of the vascular function.

U2 - 10.3109/1354750X.2013.770553

DO - 10.3109/1354750X.2013.770553

M3 - Article

C2 - 23480296

SN - 1354-750X

VL - 18

SP - 221

EP - 229

JO - Biomarkers

JF - Biomarkers

IS - 3

ER -

Real-time RT-PCR threshold cycles value for Kir6.1 from the blood correlates with parameters of vascular function: a potential for the vascular function biomarker?

Abstract

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Projects

Regulation of Subsarcolemmal ATP by Subsarcolemmal KATP Channels: Is it Important for Cardioprotection

Student theses

Determining whether plasma levels of KATP channels are related to vascular function

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