Recognition of immunogold markers in electron micrographs

Ruixuan Wang, Himanshu Pokhariya, Stephen J. McKenna, John Lucocq

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    Immunoelectron microscopy is used in cell biological research to study the spatial distribution of intracellular macromolecules at the ultrastructural level. Colloidal gold particles (immunogold markers) are commonly used to localise molecules of interest on ultrathin sections and can be visualised in transmission electron micrographs as dark spots. Quantitative analysis involves detection of the immunogold markers, and is often performed manually or interactively as part of a stereological estimation technique. The method presented in this paper automatically detects and counts immunogold markers, estimating the location, size and type of each marker. It was evaluated on single-labelled as well as double-labelled images showing markers of two different sizes. This is a first step towards automatic analysis of immunoelectron micrographs, enabling a rapid and more complete quantitative analysis than is currently practicable. (C) 2011 Elsevier Inc. All rights reserved.

    Original languageEnglish
    Pages (from-to)151-158
    Number of pages8
    JournalJournal of Structural Biology
    Volume176
    Issue number2
    DOIs
    Publication statusPublished - Nov 2011

    Keywords

    • Immunoelectron microscopy
    • Immunogold markers
    • Spot
    • Stereological approach
    • Thin sections
    • Microscopy
    • Quantitation
    • Images

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