Projects per year
Abstract
Group A Streptococcus (Strep A) is a human-exclusive bacterial pathogen killing annually more than 500,000 patients, and no current licensed vaccine exists. Strep A bacteria are highly diverse, but all produce an essential, abundant, and conserved surface carbohydrate, the Group A Carbohydrate, which contains a rhamnose polysaccharide (RhaPS) backbone. RhaPS is a validated universal vaccine candidate in a glycoconjugate prepared by chemical conjugation of the native carbohydrate to a carrier protein. We engineered the Group A Carbohydrate biosynthesis pathway to enable recombinant production using the industry standard route to couple RhaPS to selected carrier proteins within Escherichia coli cells. The structural integrity of the produced recombinant glycoconjugate vaccines was confirmed by Nuclear Magnetic Resonance (NMR) spectroscopy and mass spectrometry. Purified RhaPS glycoconjugates elicited carbohydrate-specific antibodies in mice and rabbits and bound to the surface of multiple Strep A strains of diverse M-types, confirming the recombinantly produced RhaPS glycoconjugates as valuable vaccine candidates.
Original language | English |
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Article number | 16 |
Number of pages | 14 |
Journal | NPJ Vaccines |
Volume | 10 |
Early online date | 22 Jan 2025 |
DOIs | |
Publication status | E-pub ahead of print - 22 Jan 2025 |
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Dive into the research topics of 'Recombinant production platform for Group A Streptococcus glycoconjugate vaccines'. Together they form a unique fingerprint.-
Group A Streptococcus Vaccine Development
Dorfmueller, H. (Investigator)
1/02/24 → 31/01/27
Project: Research
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Development of a universal Group A Streptococcus Glycoconjugate vaccine (joint with LSHTM)
Dorfmueller, H. (Investigator)
1/10/21 → 30/04/24
Project: Research
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Functional and Structural Studies of the Streptococcal Virulence Factor Group A Carbohydrate Biosynthesis Pathway (Sir Henry Dale Fellowship)
Dorfmueller, H. (Investigator)
1/04/16 → 30/06/22
Project: Research
Research output
- 1 Preprint
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A platform for the recombinant production of Group A Streptococcus glycoconjugate vaccines
Ajay Castro, S., Passmore, I. J., Ndeh, D., Shaw, H. A., Rudas, A., Burns, K., Thomson, S., Nagar, R., Alagesan, K., Lucas, K., Abouelhadid, S., Schwarz-Linek, U., Mawas, F., Widmalm, G., Wren, B. W. (Lead / Corresponding author) & Dorfmueller, H. C. (Lead / Corresponding author), 2 Mar 2024, BioRxiv, 34 p.Research output: Working paper/Preprint › Preprint
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