Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments

Glenn R. Masson, John E. Burke, Natalie G. Ahn, Ganesh S. Anand, Christoph Borchers, Sébastien Brier, George M. Bou-Assaf, John R. Engen, S. Walter Englander, Johan Faber, Rachel Garlish, Patrick R. Griffin, Michael L. Gross, Miklos Guttman, Yoshitomo Hamuro, Albert J.R. Heck, Damian Houde, Roxana E. Iacob, Thomas J.D. Jørgensen, Igor A. KaltashovJudith P. Klinman, Lars Konermann, Petr Man, Leland Mayne, Bruce D. Pascal, Dana Reichmann, Mark Skehel, Joost Snijder, Timothy S. Strutzenberg, Eric S. Underbakke, Cornelia Wagner, Thomas E. Wales, Benjamin T. Walters, David D. Weis, Derek J. Wilson, Patrick L. Wintrode, Zhongqi Zhang, Jie Zheng, David C. Schriemer, Kasper D. Rand

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Abstract

Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful biophysical technique being increasingly applied to a wide variety of problems. As the HDX-MS community continues to grow, adoption of best practices in data collection, analysis, presentation and interpretation will greatly enhance the accessibility of this technique to nonspecialists. Here we provide recommendations arising from community discussions emerging out of the first International Conference on Hydrogen-Exchange Mass Spectrometry (IC-HDX; 2017). It is meant to represent both a consensus viewpoint and an opportunity to stimulate further additions and refinements as the field advances.

Original languageEnglish
Pages (from-to)595-602
Number of pages8
JournalNature Methods
Volume16
Issue number7
Early online date27 Jun 2019
DOIs
Publication statusPublished - Jul 2019

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    Masson, G. R., Burke, J. E., Ahn, N. G., Anand, G. S., Borchers, C., Brier, S., Bou-Assaf, G. M., Engen, J. R., Englander, S. W., Faber, J., Garlish, R., Griffin, P. R., Gross, M. L., Guttman, M., Hamuro, Y., Heck, A. J. R., Houde, D., Iacob, R. E., Jørgensen, T. J. D., ... Rand, K. D. (2019). Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments. Nature Methods, 16(7), 595-602. https://doi.org/10.1038/s41592-019-0459-y