Recruitment of factors linking transcription and processing of pre-rRNA to NOR chromatin is UBF-dependent and occurs independent of transcription in human cells

José-Luis Prieto, Brian McStay

    Research output: Contribution to journalArticle

    105 Citations (Scopus)

    Abstract

    Efficient ribosome biogenesis requires coordination of a highly complex series of events. Early events include pre-RNA transcription, processing, and modification. Analysis in yeast has demonstrated that t-UTPs, components of the U3 snoRNA-containing pre-rRNA processing complex, are required for efficient transcription of ribosomal genes (rDNA) by RNA polymerase I (pol I). Here, we characterize human t-UTPs and establish that their ability to link transcription and pre-rRNA processing is evolutionarily conserved. The pol I transcription factor UBF binds extensively across rDNA throughout the cell cycle, resulting in a specialized form of chromatin that is the hallmark of active nucleolar organizer regions (NORs). Transcriptionally silent pseudo-NORs are ectopic, chromosomally integrated, artificial arrays that mimic this specialized chromatin structure. Pseudo-NORs sequester t-UTPs and factors linking transcription with pre-rRNA modification (Nopp140 and Treacle). Recruitment is independent of transcription, the underlying DNA sequence, and location within the nucleolus. Previously, we have demonstrated that pseudo-NORs sequester every component of the pol I transcription machinery. Taken together, these results highlight the importance of the specialized chromatin structure at active NORs in coordinating early events in ribosome biogenesis and nucleolar formation.
    Original languageEnglish
    Pages (from-to)2041-54
    Number of pages14
    JournalGenes & Development
    Volume21
    Issue number16
    DOIs
    Publication statusPublished - 1 Aug 2007

    Keywords

    • Base Sequence
    • Cell Cycle
    • Chromatin
    • DNA, Ribosomal
    • HeLa Cells
    • Humans
    • Multiprotein Complexes
    • Nuclear Proteins
    • Nucleolus Organizer Region
    • Phosphoproteins
    • Pol1 Transcription Initiation Complex Proteins
    • RNA Polymerase I
    • RNA Precursors
    • RNA Processing, Post-Transcriptional
    • RNA, Small Interfering
    • Ribonucleoproteins, Small Nucleolar
    • Transcription, Genetic

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